Publication: Histomorphometric and immunohistochemical study
of the goat omasum during prenatal development
Authors
García, A. ; Masot, Javier ; Franco, Antonio ; Gazquez, Antonio ; Redondo, E.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This work studies the morphological changes
taking place in the goat omasum during prenatal
development, using scanning electron microscope, light
microscopy and immunohistochemical analysis. A total
of 140 goat embryos and fetuses were used, from the
first stages of prenatal life until birth. Differentiation of
the omasum as a separate compartment of the primitive
gastric tube was observed at 35 days of prenatal life
([crown-rump length (CRL)] 3 cm, 23% gestation). By
38 days (CRL 4.3 cm, 25% gestation) the omasal wall
comprised three layers: an internal epithelial layer, a
middle layer of pluripotential blastemic tissue and an
external layer or serosa. Omasal laminae appeared in the
following order: primary at 38 days (CRL 4.3 cm, 25%
gestation), secondary at 50 days (CRL 7.7 cm, 33%
gestation), tertiary at 59 days (CRL 12 cm, 39%
gestation) and quaternary at 64 days (CRL 13.5 cm, 43%
gestation). Neuroendocrine cells were detected by
synaptophysin (SYP) at 52 days (CRL 8 cm, 35%
gestation), while glial cell markers (glial fibrillary acidic
protein - GFAP, and vimentin-VIM) were observed at 64
days (CRL 13.5 cm, 43% gestation) and 38 days (CRL
4.3 cm, 25% gestation), respectively. Sympathetic and
parasympathetic nerve fibers and nerve bodies were
detected via neuropeptide Y (NPY) and vasoactive
intestinal polypeptide (VIP) at 95 days (CRL 20 cm,
63% gestation). In conclusion, prenatal development of
the omasum - like that of the rumen - appears to take
place somewhat earlier in goats than in sheep or cattle,
but at a similar stage to that reported in deer.
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Citation
Histology and Histopathology, vol. 28, nº 6, (2013)
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