Publication: Expression analysis of MAC30 in human pancreatic cancer and tumors of the gastrointestinal tract
Authors
Kayed, Hany ; Kleeff, Jörg ; Ding, J. ; Hammer, J. ; Giese, T. ; Zentgraf, H. ; Büchler, M.W. ; Friess, Helmut
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Meningioma-associated protein, MAC30,
is a protein with unknown function and cellular
localization that is differentially expressed in certain
malignancies. In the present study, the expression of
MAC30 in a variety of normal and cancerous human
gastrointestinal tissues, with special emphasis on
pancreatic tissues was analyzed. Quantitative RT-PCR
was utilized to compare MAC30 expression levels. In
situ hybridization and immunohistochemistry were
carried out to localize MAC30 mRNA and protein
expression in normal and cancerous tissue samples of the
esophagus, stomach, colon and pancreas. Furthermore,
the effects of TGF-ß on the transcription of MAC30
mRNA were examined in pancreatic cancer cells.
MAC30 mRNA was expressed in a wide variety of
normal human tissues, being most abundant in testicular
and gastric tissue samples. MAC30 mRNA levels were
significantly increased in breast and colon cancer, but
significantly decreased in pancreatic and renal cancer.
TGF-ß down-regulated MAC30 mRNA levels in certain
pancreatic cancer cells. MAC30 protein was localized in
normal pancreatic tissues, mainly in acinar and islet
cells, and in normal colon, gastric and esophageal tissues
especially in the mucosal cells. MAC30 was strongly
present in tubular complexes in pancreatic cancer tissues
but weak to absent in pancreatic cancer cells of primary
tumors and metastases. In contrast, esophageal, gastric
and colon tumors displayed strong MAC30 immunoreactivity in the cancer cells. In conclusion,
MAC30 is expressed in various normal and diseased
human tissues. MAC30 up-regulation in certain tumors
and down-regulation in others suggests that this protein
plays a distinct role in human malignancies.
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