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Effects of a probiotic on the morphology and mucin composition of pig intestine

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dc.contributor.authorDesantis, Salvatore
dc.contributor.authorMastrodonato, Maria
dc.contributor.authorAccogli, Gianluca
dc.contributor.authorRossi, Giacomo
dc.contributor.authorCrovace, Alberto Maria
dc.date.accessioned2022-07-11T07:04:42Z
dc.date.available2022-07-11T07:04:42Z
dc.date.issued2019
dc.description.abstractAlthough the use of probiotics in human and animal medicine is growing, their mode of action remains poorly understood. This study examined the effects of a multi-strain probiotic (SLAB51™) on the morphology and carbohydrate composition of mucins secreted by goblet cells of intestinal crypts in growingfinishing pigs. Sections of duodenum, caecum and colon from pigs fed for 12 weeks with an orally administered control basal diet (No-Pro) or one with a probiotic blend (Pro) were processed for microscopic analysis and stained with (1) haematoxylin-eosin for structural and morphometrical investigation; (2) conventional histochemistry (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) for neutral, acidic nonsulphated, and sulphated mucin analysis; and (3) FITClabelled MAA-II and SNA lectins for α2,3- and α2,6- sialomucin identification. Compared with No-Pro samples, Pro samples displayed (1) increased goblet cell numbers in all investigated tract crypts; (2) an increase in acidic non-sulphomucins but a decrease in neutral, sulphated and α2,6-sialomucin-secreting goblet cells in the duodenum; (3) decreased crypt depth, an increase in α2,6-sialomucin secretory goblet cells, and a loss of goblet cell-secreting α2,3-sialomucins, which appeared on the apical surface of crypt fundus epithelial cells in the caecum; and (4) an increase in α2,6-sialomucinproducing goblet cells in the colon. Results suggest that treatment with SLAB51™ induces region-specific changes in the morphology and carbohydrate composition of mucins secreted along intestinal tracts of growing-finishing pigs. These changes could ameliorate the health status of the animals, which displayed higher growth performance and meat quality than controls (Tufarelli et al., 2017).es
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dc.format.extent14es
dc.identifier.citationHistology and Histopathology, Vol.34, nº9, (2019)
dc.identifier.doiDOI: 10.14670/HH-18-106
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/122263
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMucinses
dc.subjectIntestinees
dc.subjectGlycohistochemistryes
dc.subjectProbiotices
dc.subjectDietes
dc.subjectSwinees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleEffects of a probiotic on the morphology and mucin composition of pig intestinees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.34, nº9 (2019)