Publication: Seminal plasma mitigates the adverse effect of uterine fluid on boar spermatozoa
Authors
Luongo, Chiara ; Abril Sánchez, Silvia ; Hernández Cifre, José G. ; García Vázquez, Francisco A.
item.page.secondaryauthor
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1016/j.theriogenology.2019.06.018
item.page.type
info:eu-repo/semantics/article
Description
© 2019 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Theriogenology. To access the final edited and published work see https://doi.org/10.1016/j.theriogenology.2019.06.018
Abstract
After natural or artificial insemination, spermatozoa start their journey within the uterus to reach the site
of fertilization, but only few of them attain this goal. Part of this spermatozoa loss happens in the uterus,
in which uterine fluid (UF) seems to be involved. It is known from other species that UF provokes damage
to spermatozoa, which is avoided when seminal plasma (SP) is present. Therefore, the aim of the present
study was to evaluate the effect of UF on the quality of ejaculated (previously contacted with SP) and
epididymal (without previous contact with SP) boar spermatozoa analyzing motility, kinetic parameters,
viability and acrosome integrity in the presence or absence of SP over time. Three experimental groups
were evaluated in each source of spermatozoa (ejaculated and epididymal): 1) Control: spermatozoa
with 20% of SP; 2) UF: spermatozoa with 20% of UF; and 3) UF-SP: spermatozoa with 20% of SP and 20% of
UF. Total and progressive motility, kinetic parameters (VCL, VSL, VAP, LIN, STR, WOB, and BCF), viability
and acrosome damage were analyzed at 15, 60, 120 and 180 min after incubation. Total and progressive
motility decreased when ejaculated spermatozoa were incubated in UF in contrast to control and UF-SP
groups (p < 0.0007), with no differences between control and UF-SP. The VCL decreased in the UF group
compared to the control and UF-SP groups in ejaculated spermatozoa (p = 0.0002). The VSL, VAP, LIN and
STR kinetic parameters were greater when ejaculated spermatozoa were incubated in the UF-SP group
than in the UF group (all: p < 0.02). Acrosome damage increased in ejaculated and epididymal spermatozoa
incubated in the UF group compared to the control and UF-SP groups (both: p < 0.0001). Also,
the viability of epididymal spermatozoa decreased in the UF group, while it did not change in the control
and UF-SP groups (p = 0.0004). The rest of the parameters in either ejaculated or epididymal spermatozoa
did not differ between experimental groups, except for WOB when epididymal spermatozoa were
used (UF-SP higher than the control group, with UF being similar for both; p = 0.03). In conclusion, both
ejaculated and epididymal spermatozoa are affected by UF, suggesting a negative effect on their quality.
This negative effect is reduced by the presence of SP, improving the spermatozoa functionality, preserving
motility, viability and acrosome integrity.
publication.page.subject
Citation
Theriogenology, 2019, Vol. 136, pp. 28-35
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/