Publication:
Evaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environment

Simple item page
dc.contributor.authorRodríguez Lozano, Francisco Javier
dc.contributor.authorCollado-González, Mar
dc.contributor.authorLópez-García, Sergio
dc.contributor.authorGarcía Bernal, David
dc.contributor.authorMoraleda Jiménez, José María
dc.contributor.authorLozano, Adrián
dc.contributor.authorForner, Leopoldo
dc.contributor.authorMurcia, Laura
dc.contributor.authorOñate Sánchez, Ricardo Elías
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-09-25T07:17:15Z
dc.date.available2025-09-25T07:17:15Z
dc.date.copyright© 2019 International Endodontic Journal
dc.date.issued2019-03-03
dc.description.abstractAim To analyse in vitro changes in ion release and biological properties of Endocem-MTA (Maruchi, Wonju, Korea) and NeoMTA-Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs). Methodology Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and ion release was evaluated by inductively coupled plasma-mass spectrometry. Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post-test (a < 0.05). Results The MTT assay revealed non-cytotoxic effects of NeoMTA-Plus and Endocem-MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca2+ from NeoMTA-Plus and Endocem-MTA in acidic and physiological environments. Conclusions An acidic environment favoured the release of Ca2+ ions from both bioactive cements, and the cytotoxicity of these bioactive cements was low in both environments studied.
dc.formatapplication/pdf
dc.format.extent14
dc.identifier.citationInternational Endodontic Journal (2019) 52(8):1196-1209
dc.identifier.doihttps://doi.org/10.1111/iej.13107
dc.identifier.eissn1365-2591
dc.identifier.issn0143-2885
dc.identifier.urihttp://hdl.handle.net/10201/161469
dc.languageeng
dc.relationThis study was funded by the Instituto de Salud Carlos III (ISCIII) through the Spanish Net of Cell Therapy (Ter-Cel), RETICS subprogram of the I + D + I 2013-2016 Spanish National Plan, project ‘RD16/0011/0001’ (to JMM), funded by ISCIII and co-founded by FEDER.
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.13107
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAcidic environment
dc.subjectNeo-MTA Plus
dc.subjectAcidic environment
dc.subjectBioactive endodontic cements
dc.subjectBiological properties
dc.subjectIon release
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleEvaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environment
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
relation.isAuthorOfPublication32059c24-bbdc-4ba3-a281-72ad733fc960
relation.isAuthorOfPublicationba32f760-d55c-40a7-9452-62dfd47cbeaf
relation.isAuthorOfPublication2372b160-a380-45dd-835a-6f377fbeaf97
relation.isAuthorOfPublication0d591113-ba56-4833-a0fa-6a44b4720ec2
relation.isAuthorOfPublicationdd1715f8-fe64-4ed6-8cf2-dc327b3020e6
relation.isAuthorOfPublication.latestForDiscovery32059c24-bbdc-4ba3-a281-72ad733fc960
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Int End J_Rodriguez_digitum.pdf
Size:
4.94 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos