Publication:
Evaluation of refrigerated platelet concentrates supplemented with low doses of second messenger effectors

Simple item page
dc.contributor.authorPérez Ceballos, Elena
dc.contributor.authorRivera Pozo, José
dc.contributor.authorLozano Almela, María Luisa
dc.contributor.authorCandela, M.J.
dc.contributor.authorCorral de la Calle, Javier
dc.contributor.authorGuerrero López, José Antonio
dc.contributor.authorVicente García, Vicente
dc.contributor.departmentMedicina Interna
dc.date.accessioned2026-02-28T09:29:49Z
dc.date.available2026-02-28T09:29:49Z
dc.date.copyright© 2004 Blackwell Publishing Ltd,
dc.date.issued2004-07-27
dc.description.abstractWith the goal of producing haemostatically effective platelet concentrates (PCs) with a longer shelf-life, we aimed to identify a simple combination of platelet inhibitors, with a low pharmacological load, which could avoid the unacceptable loss of platelets stored under refrigerated conditions. PCs stored with different combinations of second messenger effectors were analysed at days 5, 10 and 15 of storage and compared with those supplemented with ThromboSol – a combination of six platelet inhibitors that protects cells from cold damage. The following parameters were analysed: platelet counts, biochemical parameters (glucose, pH, bicarbonate, lactate), cell lysis (lactic dehydrogenase, LDH), membrane glycoproteins (GPs), platelet aggregation, fibrinogen binding and hypotonic shock response. We characterized the combination of amiloride and sodium nitroprusside (at 1/2 the dose included in ThromboSol). This was found to be similar to ThromboSol and superior to nontreated units in the prevention of cold-induced platelet aggregation at day 15 of storage (maintenance of 78% and 80% of initial platelet counts, respectively), preservation of GPIba (11% and 12% better maintenance of mean fluorescence intensity compared with control units, respectively), and reduced cell lysis (13% and 11% decrease in supernatant LDH, respectively). The reduced pharmacological load with the identified solution compared with ThromboSol is an argument in favour of the potential use of these agents when designing strategies to improve PC storage.
dc.formatapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/j.1365-2257.2004.00619.x
dc.identifier.eissn1751-553X
dc.identifier.issn1751-5521
dc.identifier.urihttp://hdl.handle.net/10201/216381
dc.languageeng
dc.publisherWiley
dc.relationFondo de Investigaciones Sanitarias (FIS 01/1689).
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/j.1365-2257.2004.00619.x
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectThrombosol
dc.subjectCold storage
dc.subjectPlatelet concentrate
dc.subjectAmiloride
dc.subjectSodium nitroprusside
dc.subject.odsObjetivo 3: Salud
dc.titleEvaluation of refrigerated platelet concentrates supplemented with low doses of second messenger effectors
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication41cd0559-4e25-499c-9f9c-9fe51a342334
relation.isAuthorOfPublication4b8d7b3a-4e01-4820-b6e1-970f8b104f33
relation.isAuthorOfPublicationee79a18b-41e0-496f-8af5-70584cfa100d
relation.isAuthorOfPublicationb5482ef9-c053-4abc-b51d-9daf5c588d07
relation.isAuthorOfPublication099fb9a8-9bae-43fb-be04-b8ea37502882
relation.isAuthorOfPublication3cee2690-9910-4fc5-a0e7-db3696d41388
relation.isAuthorOfPublication.latestForDiscovery41cd0559-4e25-499c-9f9c-9fe51a342334
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2004_Clin_Lab_Haem.pdf
Size:
188.58 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos