Publication: Cellular localization of fibroblast growth factor 2 , FGF,-2 in benign prostatic hyperplasia
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Date
2000
Authors
Sinowatz, F. ; Schams, D. ; Einspanier, R. ; Arnold, G. ; Pfeffel, M. ; Temmim-Bakers, L. ; Amselgruber, W. ; Plendl, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Fibroblast growth factor 2 (FGF-2, basic
fibroblast growth factor) has been reported to be
elevated in tissues from benign prostatic hyperplasia
(BPH), the most frequent neoplastic disease in aging
men. This suggests that FGF-2 may play a significant
role in the development of BPH. In this study the
cellular distribution pattern of FGF-2 in tissues from
BPH has been investigated by immunohistochemical and
molecular biological methods. Radioimmunoassay
revealed high concentrations of FGF-2, ranging between
450 and 950 ng per g tissue. Immunoblots confirmed the
presence of a 18 kDa FGF-2 in tissue extracts. By
immunohistochemistry done with a polyclonal antibody
to recombinant FGF-2 on paraffin sections, FGF-2 was
localized in fibroblasts, endothelial cells and smooth
muscle cells of tissue samples of BPH. Nuclei of these
cells were labelled distinctly. Moreover the cytoplasm of
smooth muscle cells was labelled moderately. No
immunostaining was seen in prostatic epithelium. Nonradioactive
in situ hybridization with digoxygeninlabelled
oligonucleotides revealed the presence of
mRNA for FGF-2 in smooth muscle cells of the prostatic
stroma. These results provide evidence that FGF-2 may
be produced locally in the human prostate as a stromaspecific
mitogen and may play a causal role in the
development of BPH.
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