Publication: Prodigiosin induces cell death and morphological changes indicative
of apoptosis in gastric cancer cell line HGT-1
Authors
Díaz Ruiz, C. ; Montaner, B. ; Pérez-Tomás, R.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Gastric cancer is one of the most frequent
malignancies and its treatment is far from satisfactory.
The challenge to oncologists is the characterization of
novel chemical entities with greater effectiveness.
Prodigiosin is a red pigment produced by various
bacteria including Serratia rnarcescens. Here we
characterize the apoptotic action of prodigiosin in human
gastric carcinoma celi line (HGT-1). Cells were assayed
by the MTT assay, fragmentation pattern of DNA,
Hoechst 33342 staining and study of actin microfilament
architecture. Treatment of these cells with prodigiosin
showed a constant decrease in viability by apoptosis.
Morphological analysis of prodigiosin-treated cells
demonstrated that prodigiosin induces cell shrinkage,
chromatin condensation, reorganization of actin
microfilament architecture, and detachment of cells from
the cell culture substrate. Altogether these results
suggest that prodigiosin induces apoptosis in HGT-1
human gastric cancer cells and raises the possibility of
its use as a new chemotherapeutic drug.
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