Publication: Reverse lectin histochemistry: Design and application of glycoligands for detection of cell and tissue lectins
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Date
1993
Authors
Gabius, H.J. ; Gabius, S. ; Zemlyanukhina, T.V. ; Bovin, N.V. ; Brinck, U. ; Danguy, A. ; Joshi, S.S. ; Kaiser, K. ; Schottelius, J. ; Sinowatz, F. ; Tietze, L.F. ; Vidal-Vanaclocha, F. ; Zanetta, J.P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Plant and invertebrate lectins are valuable
cyto- and histological tools for the localization
of defined carbohydrate determinants. The welldocumented
ubiquitous occurrence of sugar receptors
encourages functional considerations. Undoubtedly,
analysis of the presence of vertebrate lectins in tissues
and cells is required to answer the pertinent and
tempting question on the physiological relevance of
protein (1ectin)-carbohydrate recognition in situ. Carrierimmobilized
glycoligands, derived from custom-made
chemical synthesis, enable the visualization of respective
binding sites. Histochemically inert proteins or synthetic
polymers with appropriate functional groups are suitable
carrier molecules for essential incorporation of ligand
and label. The resulting neoglycoconjugates can track
down tissue receptors that are neither impaired by
fixation procedures nor blocked by endogenous highaffinity
ligands. Lectins, especially the receptors of the
tissue under investigation (endogenous lectins), and
appropriately tailored immobilized glycoligands or
lectin-specific antibodies (when available) are
complementary tools to test the attractive hypothesis that
diverse, functionally relevant glycobiological processes
within or between cells are operative. Concomitant
evaluation of both sides of lectin histochemistry, namelylectins as tools and lectins as functionally important
molecules in situ, will indubitably render desired
progress amenable in our often still fragmentary
understanding of the importance of tissue lectin and
glycoconjugate expression and its regulation.
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