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The regulation mechanism of apoptosis by visfatin in the mesenteric lymph nodes of LPS-treated rats

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dc.contributor.authorXiao, Ke
dc.contributor.authorZhou, Ying
dc.contributor.authorYuan, Huai Rui
dc.contributor.authorCui, Lu
dc.contributor.authorRehman, Zia ur
dc.contributor.authorAnsari, Abdur Rahman
dc.contributor.authorYang, Zhi
dc.contributor.authorPeng, Ke Mei
dc.contributor.authorSong, Hui
dc.date.accessioned2021-10-27T07:49:30Z
dc.date.available2021-10-27T07:49:30Z
dc.date.issued2016
dc.description.abstractVisfatin is an adipocytokine displaying multiple functional properties, which plays a role in the regulation of cell apoptosis and inflammation by an as yet unidentified mechanism. The aim of the present study was to determine if visfatin is involved in apoptosis pathway induced by LPS in rat Mesenteric lymph nodes (MLNs). Experimental rats were divided into four groups and MLNs samples were collected from each group. The morphological changes of the MLNs were examined by histological imaging. CD68 and ENPP1 were detected with immunohistochemistry and Western Blot. Apoptosis was evaluated with TUNEL and Flow Cytometry, the mRNA levels of the apoptosisrelated genes were detected by qRT-PCR, and the protein levels of the apoptotic-related factors were detected by western blot. The main results showed that visfatin could significantly increase the macrophages in MLNs and prevent cell apoptosis from LPS-induced mesenteric lymph nodes, activate apoptotic signaling pathways and regulate the mRNA levels of the apoptosis-related genes. Visfatin had a pro-apoptotic effect on normal MLNs, whereas it exerted an anti-apoptotic effect during LPSinduced cell apoptosis in rat MLNs. In short, visfatin plays a dual role in the apoptosis in rat MLNs, which is mediated by both the mitochondrial apoptotic pathway and the death-receptor apoptotic pathway.es
dc.formatapplication/pdfes
dc.format.extent14es
dc.identifier.citationHistology and Histopathology, Vol.31, nÂş9, (2016)
dc.identifier.doiDOI: 10.14670/HH-11-731
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/113404
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de BiologĂ­a Celular e HistologĂ­aes
dc.relationSin financiaciĂłn externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectVisfatines
dc.subjectMLNses
dc.subjectInflammationes
dc.subjectLPSes
dc.subjectApoptosis pathwayes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - PatologĂ­a. Medicina clĂ­nica. OncologĂ­aes
dc.titleThe regulation mechanism of apoptosis by visfatin in the mesenteric lymph nodes of LPS-treated ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.31, nÂş9 (2016)