Publication: Circ_0085616 contributes to the radio-resistance and progression of cervical cancer by targeting miR-541-3p/ARL2 signaling
Authors
Tang, Yan ; Zhou, Lei ; Liu, Lili
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-541
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info:eu-repo/semantics/article
Description
Abstract
Background. Circular RNAs (circRNAs)
play crucial regulatory roles in cancer progression and
the development of radio-resistance. Here, we intended
to explore the role of circ_0085616 in cervical cancer
progression and its associated mechanism.
Methods. Colony formation assay was employed to
analyze the radio-resistance and proliferation of cervical
cancer cells. Cell proliferation ability was also assessed
by 5-ethynyl-2’-deoxyuridine (EdU) assay. Cell
apoptosis was analyzed by flow cytometry. Tube
formation assay was performed to analyze cell
angiogenesis ability. Transwell assays were conducted to
measure cell migration and invasion abilities. Dualluciferase reporter assay was utilized to verify the target
relationships. Xenograft mice model was used to analyze
the role of circ_0085616 in tumor growth in vivo.
Results. Circ_0085616 expression was elevated in
cervical cancer tissues and cell lines. Circ_0085616
interference suppressed the radio-resistance,
proliferation, tube formation, migration, and invasion
and elevated the apoptosis rate of cervical cancer cells.
Circ_0085616 acted as a sponge for microRNA-541-3p
(miR-541-3p), and miR-541-3p was negatively regulated
by circ_0085616 in cervical cancer cells. Circ_0085616
absence-induced changes in the behaviors of cervical
cancer cells were largely overturned by anti-miR-541-
3p. miR-541-3p negatively regulated ADP ribosylation
factor like GTPase 2 (ARL2) expression by binding to
its 3’ untranslated region (3’UTR). miR-541-3p mimicinduced effects were largely reversed by pcDNA-ARL2
in cervical cancer cells. Circ_0085616 positively
regulated ARL2 expression by sequestering miR-541-3p.
Circ_0085616 absence significantly inhibited the tumor
growth in vivo.
Conclusion. Circ_0085616 contributed to the radioresistance and progression of cervical cancer partly
through mediating the miR-541-3p/ARL2 axis.
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Citation
Histology and Histopathology Vol. 38, nº5 (2023)
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