Publication:
Netrin-1/DCC signaling differentially regulates the migration of Pax7, Nkx6.1, Irx2, Otp and Otx2 cell populations in the developing interpeduncular nucleus

Simple item page
dc.contributor.authorGarcía-Guillén, Isabel M.
dc.contributor.authorMorales-Delgado, Nicanor
dc.contributor.authorAndrés, Belén
dc.contributor.authorPuelles López, Luis
dc.contributor.authorLópez-Bendito, Guillermina
dc.contributor.authorAlonso Fuentes, Antonia
dc.contributor.authorAroca Tejedor, Pilar
dc.contributor.authorMarín San Leandro, Faustino
dc.contributor.departmentAnatomía Humana y Psicobiología
dc.date.accessioned2021-03-24T07:54:36Z
dc.date.available2021-03-24T07:54:36Z
dc.date.created2020
dc.date.issued2020-10-20
dc.description© 2020. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published version of a Published Work that appeared in final form in Frontiers in Cell and Developmental Biology. To access the final edited and published work see https://doi.org/10.3389/fcell.2020.588851
dc.description.abstractThe interpeduncular nucleus (IPN) is a hindbrain structure formed by three main subdivisions, the prodromal (Pro) domain located at the isthmus (Ist), and the rostral and caudal interpeduncular domains (IPR, IPC) within rhombomere 1 (r1). Various cell populations can be detected in the IPN through the expression of the Nkx6.1, Otp, Otx2, Pax7, and/or Irx2 transcription factors. These cell populations follow independent dorsoventral tangential and radial migratory routes targeting the ventral paramedian region of Ist and r1. Here we set out to examine the influence of the Netrin-1/DCC pathway on these migrations, since it is known to regulate other processes of neuronal migration in the brain. To this end, we analyzed IPN development in late gestational wild-type and DCC-/- mice, using mainly in situ hybridization (ISH) to identify the cells expressing each of the aforementioned genes. We found that the migration of Nkx6.1 + and Irx2 + cells into the Pro domain was strongly disrupted by the loss of DCC, as occurred with the migration of Pax7 +, Irx2 +, and Otp + cells that would normally form the IPR. In addition, there was mild impairment of the migration of the Pax7 + and Otx2 + cells that form the IPC. These results demonstrate that the Netrin-1/DCC signaling pathway is involved in the migration of most of the IPN populations, mainly affecting those of the Pro and IPR domains of this nucleus. There are psychiatric disorders that involve the medial habenula (mHb)-IPN system, so that this experimental model could provide a basis to study their neurodevelopmental etiology.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationFrontiers in Cell and Developmental Biology, 2020 8:588851 Front. Cell Dev. Biol..
dc.identifier.doi10.3389/fcell.2020.588851
dc.identifier.issn2296-634X
dc.identifier.urihttp://hdl.handle.net/10201/105581
dc.languageenges
dc.publisherFrontiers Media
dc.relationThis work was funded by the Seneca Foundation to LP(Autonomous Community of Murcia, Excellency Researchcontract, reference: 19904/GERM/15; 5672 Fundación Séneca;project name: Genoarchitectonic Brain Development andApplications to Neurodegenerative Diseases and Cancer)and by the Knowledge Generation Program of the SpanishGovernment to GL-B (PGC2018-096631-B-I00). IG-G wasrecipient of a predoctoral fellowship of the FPU program fromthe University of Murcia.es
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fcell.2020.588851/fulles
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectIPNes
dc.subjectInterpeduncular nucleuses
dc.subjectProdromales
dc.subjectInterpeduncular rostrales
dc.subjectInterpeduncular caudales
dc.subjectIsthmuses
dc.subjectRrhombomere 1es
dc.subjectMedial habenulaes
dc.subjectRhombomere 2es
dc.subjectRhombomere 1 rostrales
dc.subjectRhombomere 1 caudales
dc.subject.otherCDU::5 - Ciencias puras y naturaleses
dc.titleNetrin-1/DCC signaling differentially regulates the migration of Pax7, Nkx6.1, Irx2, Otp and Otx2 cell populations in the developing interpeduncular nucleuses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicatione6582236-1657-4fd1-8c98-d549ab609b91
relation.isAuthorOfPublication39981627-8a1e-4c85-b05b-af14ef212804
relation.isAuthorOfPublicationf2e3d6ed-ce98-4d29-9a0e-6f639f216e96
relation.isAuthorOfPublication.latestForDiscoverye6582236-1657-4fd1-8c98-d549ab609b91
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
García-Guillen et al-2020.pdf
Size:
5.34 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.39 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos