Publication: Distribution of cytoskeletal and adhesion proteins in adult zebrafish skeletal muscle
Authors
Câmara-Pereira, E.S. ; Campos, L.M. ; Vannier-Santos, M.A. ; Mermelstein, C.S. ; Costa, M.L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The organization of cytoskeletal and
adhesion proteins in skeletal muscle is critical for its
contractile function. Zebrafish has become a paramount
model for studies of vertebrate biology, including
muscle. However, only a few studies have been
published using immunolabeling to specifically localize
proteins in adult zebrafish muscle. To fully appreciate
the distribution of cytoskeletal and adhesion proteins,
and therefore to better correlate the adult muscle with its
myogenesis, we used indirect immunofluorescence
microscopy of frozen adult zebrafish skeletal muscle
sections. Here we describe the fish muscle cytoskeletal
architecture and location of the major myofibrillar
proteins desmin, alpha-actinin, myosin, titin, troponin,
tropomyosin and nebulin, the adhesion proteins vinculin
and paxillin, and the extracellular matrix proteins
laminin and fibronectin. Electron microscopical analysis
in ultra-thin sections of adult zebrafish skeletal muscle
showed bundles of collagen fibers and fibroblastic cells
in the extracellular space of the myosepta.
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