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Oxidative stress markers 8-OHdG, Trx-1, and Prx6 are expressed in seminoma and are connected to TXNDC2, 3, and 6 expression levels

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dc.contributor
dc.contributor.authorRantala Inka
dc.contributor.authorTeppo Hanna-Riikka
dc.contributor.authorHändelin Jonas
dc.contributor.authorKemppainen Janette
dc.contributor.authorOllikainen Riina K.
dc.contributor.authorKuittinen Outi
dc.contributor.authorKuusisto Milla E.L.
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-10-23T06:30:02Z
dc.date.available2025-10-23T06:30:02Z
dc.date.issued2025
dc.description.abstractTestis-specific thioredoxins (TXNDCs) and oxidative stress have not been studied before in seminoma. We studied the immunohistochemical (IHC) expression of 8-OHdG, Prx6, Trx-1, TXNDC2, 3, and 6 in 25 testicular seminoma and 24 control samples. We retrospectively collected patient details and treatments from hospital records and combined them with IHC results. In normal testis, IHC expression of 8-OHdG, Prx6, TXNDC2, and 3 was higher than in seminoma samples (p<0.001 each), and the absence of n8-OHdG (p=0.002) and nTXNDC2 expression (p=0.044) was clinically associated with elevated lactate dehydrogenase levels. On the contrary, Trx-1 and TXNDC6 were over-expressed in seminoma samples (p=0.035 and p<0.001, respectively), and TXNDC6 was negatively associated with age over 40 (p=0.036). Concordant with the IHC expression, PRDX6 mRNA levels were downregulated with a fold change of -2517.4 (p<0.001), and TXN mRNA levels were upregulated with a fold change of 11637.4 (p=0.008) in seminoma samples compared with healthy controls. The oxidative stress markers studied had a correlation between the nuclear and cytoplasmic localization, a mutual correlation in expression between different markers, and mutual protein-protein interactions according to STRING Enrichment analysis. Our results show that oxidative stress markers and their expression in seminoma differ from that in normal testis tissue. Trx-1 and cTXNDC6 are seemingly overexpressed in seminoma, which might indicate their relevance in seminoma biology
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dc.format.extent9
dc.identifier.citationHistology and Histopathology, volúmen 40, nº 10 (2025)
dc.identifier.doihttps://doi.org/10.14670/HH-18-898
dc.identifier.eissn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/168269
dc.languageeng
dc.publisherUniversidad de Murcia, Departamento de Histología e Histopatología
dc.relationSin financiación externa a la Universidad
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectOxidative stress
dc.subjectSeminoma
dc.subjectTestis-specific thioredoxins
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleOxidative stress markers 8-OHdG, Trx-1, and Prx6 are expressed in seminoma and are connected to TXNDC2, 3, and 6 expression levels
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublication
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Histology and histopathology Vol.40,nº10 (2025)