Publication: TenascinC and type I and III collagen expression in total Achilles tendon rupture. An immunohistochemical study
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Date
2009
Authors
Pajala, Ari ; Melkko, Jukka ; Leppilahti, Juhana ; Ohtonen, Pasi ; Soini, Ylermi ; Risteli, Juha
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Tendon tissue degeneration and changes in
collagen composition play a role in spontaneous rupture
of the human Achilles tendon. Tenascin-C has been
shown to be present in the tissue pathology and changes
in tissue loading. We made an immunohistological study
of the expression of tenascin-C and type I and III
collagens in ruptured human Achilles tendons.
METHODS: Three tissue samples in ten individuals, one
from the Achilles tendon rupture and two control
samples from four and sixteen centimeters proximal in
same tendon were collected at surgery. The specimen
were fixed and labelled with specific antibodies to type I
and III procollagens (PICP, PINP and PIIINP), mature
type III collagen (IIINTP) and tenascin-C. The amount
of reacting tissue was evaluated visually and graded on a
semiquantitative scale. RESULTS: No difference in the
expression of tenascin-C was found between the sites.
Instead, mature type III collagen content (p=0.008) and
type III collagen synthesis (p=0.016) were significantly
increased at the rupture site relative to the control site 2.
The amount of newly synthesized type I collagen (PINP,
PICP) was relatively high at all sites, as expected.
CONCLUSION: The expression of type III collagen is
increased at the rupture site in the human Achilles
tendon, but that of tenascin-C remains unchanged. This
finding supports a tissue composition alteration
background for Achilles tendon rupture, while the role
of mechanical loading at the rupture site remains
controversial.
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