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MLH1 and MSH2 expression in endometrial cancer - microscopic and computer assessment of immunohistochemical method

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dc.contributor.authorRogaliński Jan
dc.contributor.authorPrzewoźny Stanisław
dc.contributor.authorDe Mezer Mateusz
dc.contributor.authorMarkowska Anna
dc.contributor.authorMarkowska Janina
dc.contributor.authorŻurawski Jakub
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-10-15T12:06:21Z
dc.date.available2025-10-15T12:06:21Z
dc.date.issued2025
dc.description.abstractObjective. We aimed to compare MLH1 and MSH2 protein expression and Mismatch repair (MMR) status with clinical data: diagnosis, grading, and staging. Moreover, we wanted to assess any correlation between two immunohistochemical assessments: classic microscopic and computer analysis performed by a calibrated program. Materials and methods. Our studies were conducted on 95 cases of endometrial cancer. For each, we performed H+E staining and immunohistochemistry (IHC) of two MMR status proteins: MLH1 and MSH2. Two independent researchers assessed IHC on a 0 to ++++ scale. We classified cases as MMR-deficient based on the microscopic assessment of the absence of expression of at least one protein. For computer analysis, we used Olympus cellSens software to measure the positive IHC reaction area in μm2 in five fields of vision. Results. Despite using two different assessment methods, we did not identify any statistically significant relationship between diagnosis, grading, staging, MMR protein expression, and MMR status. However, we found a strong correlation between computer analysis and semi-quantitative microscopic assessment (r=0.59 for MLH1 and r=0.76 for MSH2; p<0.001 for both). Furthermore, we revealed that computer measurement of the expression area could be a good objective prediction test for microscopic analysis (p<0.001). Conclusion. We did not find a relationship between MMR status and grading, staging, or diagnosis. However, we present a novel approach to immunohisto-chemical assessment using computer analysis. It allows us to carry out more objective and accurate studies with the IHC method
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dc.format.extent10
dc.identifier.citationHistology and Histopathology, Volúmen 40, nº 9 (2025)
dc.identifier.doihttps://doi.org/10.14670/HH-18-884
dc.identifier.eissn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/166629
dc.languageeng
dc.publisherUniversidad de Murcia, Departamento de Histología e Histopatología
dc.relationSin financiación externa a la Universidad
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMMR
dc.subjectMismatch repair deficiency
dc.subjectLynch syndrome
dc.subjectImmunohistochemistry,
dc.subjectOlympus cellSens
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleMLH1 and MSH2 expression in endometrial cancer - microscopic and computer assessment of immunohistochemical method
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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Histology and histopathology Vol.40, nº9 (2025)