Publication: Histopathological features and modulation of type IV collagen expression i nd uced by Pseodomonas aeruginosa
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Date
2001
Authors
Baroni, A. ; Gorga, F. ; Baldi, A. ; Perfetto, B. ; Paoletti, I. ; Russo, A. ; Lembo, L. ; Rossano, F.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Background: Pseudomonas aeruginosa (l?
aeruginosa) is responsible for serious infections in the
immunocompromised host. Many skin lesions induced
by P. aeruginosa have been described. Few
investigations have been performed on the local action
of i? aeruginosa components. Objectives: To shed light
on the "in vivo" activity of lipopolysaccharide (LPS) and
porins extracted from i? aeruginosa, by verifying their
effects after inoculation in mouse skin through the
observation of histological changes and
immunohistochemical expression of collagen IV.
Results: Both substances were able to induce a similar
inflammatory process and a characteristic reversible
change in collagen IV distribution. Interestingly, a
fibroblast increase was observed at 24 h in the skin
treated with porins, while it appeared later in the skin
treated with LPS. Besides these changes, porins
particularly increased collagen edema, together with
disgregation of hypodermal structures. Moreover "in
vitro", porins were able to stimulate fibloblasts 3T3 to
convert 72 kDa type IV collagenase into the activated 62
kDa form and to release the 92 kDa collagenase.
Conclusion: LPS and porins, released by Gram-negative
bacteria during cell growth and lysis, interact with the
host at target cells, such as keratinocytes, fibroblasts and
immunocompetent cells, thus contributing significantly
to the pathogenesis of l? aeruginosa skin infections.
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