Publication: Multicolor FISH probe sets and their applications
Authors
Liehr, T. ; Starke, H. ; Weise, A. ; Lehrer, H. ; Claussen, U.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Multicolor fluorescence in situ hybridization
(FISH) assays are nowadays indispensable for a precise
description of complex chromosomal rearrangements.
Routine application of such techniques on human
chromosomes started in 1996 with the simultaneous use
of all 24 human whole chromosome painting probes in
multiplex-FISH (M-FISH) and spectral karyotyping
(SKY). Since then different approaches for chromosomal
differentiation based on multicolor-FISH (mFISH)
assays have been described. Predominantly, they have
been established to characterize marker chromosomes
identified in conventional banding analysis. Their
characterization is of high clinical impact and is the
requisite condition for further molecular investigations
aimed at the identification of disease-related genes. Here
we present a review on the available mFISH methods
including their advantages, limitations and possible
applications.
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