Evolution of the inflammatory response in vertebrates: fish TNF-α is a powerful activator of endothelial cells but hardly activates phagocytes

Mulero Méndez, Iván; López-Muñoz, Azucena; Renshaw, Stephen A.; Meseguer Peñalver, J.; Mulero Méndez, Victoriano Francisco; Sepulcre Cortés, María Pilar; Roca Soler, Francisco José

Publication:
Evolution of the inflammatory response in vertebrates: fish TNF-α is a powerful activator of endothelial cells but hardly activates phagocytes

dc.contributor.author	Mulero Méndez, Iván
dc.contributor.author	López-Muñoz, Azucena
dc.contributor.author	Renshaw, Stephen A.
dc.contributor.author	Meseguer Peñalver, J.
dc.contributor.author	Mulero Méndez, Victoriano Francisco
dc.contributor.author	Sepulcre Cortés, María Pilar
dc.contributor.author	Roca Soler, Francisco José
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2025-01-29T16:48:28Z
dc.date.available	2025-01-29T16:48:28Z
dc.date.created	2008-10-01
dc.description	© 2008 by The American Association of Immunologist. This document is the Published version of a Published Work that appeared in final form in The Journal of Immunology. To access the final edited and published work see https://doi.org/10.4049/jimmunol.181.7.5071
dc.description.abstract	TNF-α is conserved in all vertebrate classes and has been identified in all taxonomic groups of teleost fish. However, its biological activities and its role in infection are largely unknown. Using two complementary fish models, gilthead seabream and zebrafish, we report here that the main proinflammatory effects of fish TNF-α are mediated through the activation of endothelial cells. Thus, TNF-α promotes the expression of E-selectin and different CC and CXC chemokines in endothelial cells, thus explaining the recruitment and activation of phagocytes observed in vivo in both species. We also found that TLR ligands, and to some extent TNF-α, were able to increase the expression of MHC class II and CD83 in endothelial cells, which might suggest a role for fish endothelial cells and TNF-α in Ag presentation. Lastly, we found that TNF-α increases the susceptibility of the zebrafish to viral (spring viremia of carp virus) and bacterial (Streptococcus iniae) infections. Although the powerful actions of fish TNF-α on endothelial cells suggest that it might facilitate pathogen dissemination, it was found that TNF-α increased antiviral genes and, more importantly, had little effect on the viral load in early infection. In addition, the stimulation of ZF4 cells with TNF-α resulted in increased viral replication. Together, these results indicate that fish TNF-α displays different sorts of bioactivity to their mammalian counterparts and point to the complexity of the evolution that has taken place in the regulation of innate immunity by cytokines.	es
dc.format	application/pdf	es
dc.format.extent	12	es
dc.identifier.citation	J Immunol (2008) 181 (7): 5071–5081
dc.identifier.doi	https://doi.org/10.4049/jimmunol.181.7.5071
dc.identifier.issn	Print: 0022-1767
dc.identifier.issn	Electronic: 1550-6606
dc.identifier.uri	http://hdl.handle.net/10201/149670
dc.language	eng	es
dc.relation	Spanish Ministry of Education and Science (Grant BIO2005-05078 to V.M.) and Fundación Séneca-Murcia and University of Murcia (fellowships to I.M. and A.L.-M., respectively)	es
dc.relation.publisherversion	https://journals.aai.org/jimmunol/article/181/7/5071/84275/Evolution-of-the-Inflammatory-Response-in
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Phylogeny	es
dc.subject	Inflammatory response	es
dc.subject	Fish	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.title	Evolution of the inflammatory response in vertebrates: fish TNF-α is a powerful activator of endothelial cells but hardly activates phagocytes	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	369f9ade-8896-4d91-84c5-e3f94a526070
relation.isAuthorOfPublication	508910a4-32ba-4c84-8616-ea6c14c4f883
relation.isAuthorOfPublication	7f46a88a-1ff4-4f80-948e-7d636b80e75d
relation.isAuthorOfPublication.latestForDiscovery	369f9ade-8896-4d91-84c5-e3f94a526070