Publication: Aspergillus fumigatus causes in vitro electrophysiological and morphological
modifications in human nasal epithelial cells
Authors
Botterel, F. ; Cordonnier, C. ; Barbier, V. ; Wingerstmann, L. ; Liance, M. ; Coste, A. ; Escudier, E. ; Bretagne, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The role of the airway epithelium in the
development of invasive aspergillosis in
immunocompromised hosts has rarely been studied
although patients at risk for this infection frequently
have epithelial damage. We developed an in vitro model
of primary culture of human nasal epithelial cells
(HNEC) in air-liquid interface, which allows epithelial
cell differentiation and mimics in vivo airway
epithelium. We subsequently tested 7-day and 24-hour
Aspergillus fumigatus filtrates on the apical side of
HNEC to know whether A. fumigatus, the main species
responsible for invasive aspergillosis, produces specific
damage to the epithelial cells. The results were
compared with those obtained with non-pathogenic
filamentous fungi. Seven-day culture filtrates of A.
fumigatus and Penicillium chrysogenum induced
electrophysiological modifications whatever the fungus
tested. In contrast, only 24-hour A. fumigatus filtrates
induced a specific decrease in transepithelial resistance,
hyperpolarization of the epithelium, and cytoplasmic
vacuolization of HNEC compared with both A. niger and
Penicillium chrysogenum. The inhibition of the A.
fumigatus effects with amiloride suggests that the 24-
hour fungal filtrate acts through sodium channels of
HNEC. These early modifications of the epithelial cells
could facilitate colonization of the airways by A.
fumigatus. To know whether the molecules involved are
specific to A. fumigatus or simply produced more rapidly
than by other filamentous fungi warrants further investigation. In this perspective, the primary culture of
HNEC represents a suitable model to study the interactions between airway epithelial cells and A.
fumigatus.
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