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First description of an in vitro culture system for Eimeria ovinoidalis macromeront formation in primary host endothelial cells

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dc.contributor.authorCarrau Garreta, Tessa
dc.contributor.authorMachado Ribeiro Silva, Liliana
dc.contributor.authorPérez, David
dc.contributor.authorRuiz de Ybáñez Carnero, María del Rocío
dc.contributor.authorTaubert, Anja
dc.contributor.authorHermosilla, Carlos
dc.contributor.departmentSanidad Animal
dc.date.accessioned2024-03-14T11:35:34Z
dc.date.available2024-03-14T11:35:34Z
dc.date.issued2016-05-25
dc.description©2016. This document is the Published, version of a Published Work that appeared in final form in Parasitology International. To access the final edited and published work see http://dx.doi.org/10.1016/j.parint.2016.05.003
dc.description.abstractThe apicomplexan parasite Eimeria ovinoidalis is distributed worldwide and causes clinical ovine coccidiosis. As one of the most pathogenic species in sheep, the principal clinical sign is profuse diarrhoea in young animals, which leads to important economic losses in the ovine industry. We here aimed to establish an in vitro culture system for the development of E. ovinoidalis macromeronts, as no suitable systems are currently available for any ovine Eimeria species. Faecal samples containing more than 90% of E. ovinoidalis oocysts were collected from naturally infected lambs and ewes in Murcia Region (Spain). E. ovinoidalis oocysts were collected, left to sporulate in potassium dichromate and stored at 4 °C until further studies were conducted. Moreover, a suitable excystation protocol was effectively established, resulting in the release of viable sporozoites, which were allowed to infect primary bovine umbilical vein endothelial cells (BUVEC) and permanent bovine colonic epithelial cells (BCEC). In vitro first merogony was successfully accomplished exclusively in BUVEC leading to macromeront formation (up to 100 μm) and the release of fully developed and viable merozoites I stages. Given that we were able to establish a suitable in vitro system for the first merogony of such pathogenic Eimeria species in sheep, advances might be further made not only on studies regarding the control of ovine coccidiosis, such as drug screenings, but also on the better understanding of molecular parasite–host cell interactions as already demonstrated for other ruminant Eimeria species.
dc.formatapplication/pdfes
dc.format.extent4
dc.identifier.doihttp://dx.doi.org/10.1016/j.parint.2016.05.003
dc.identifier.eisbnParasitology International 65 (2016) 516–519es
dc.identifier.issnPrint: 1383-5769
dc.identifier.urihttp://hdl.handle.net/10201/140199
dc.languageenges
dc.publisherElsevier
dc.relationSin financiación externa a la universidades
dc.rightsinfo:eu-repo/semantics/restrictedAccesses
dc.subjectEimeria ovinoidalises
dc.subjectIn vitro culture systemes
dc.subjectOvinees
dc.subjectBUVECes
dc.subjectFirst merogonyes
dc.titleFirst description of an in vitro culture system for Eimeria ovinoidalis macromeront formation in primary host endothelial cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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