Evaluation of immune-related parameters in acellular and cellular fractions  in male and female of Atlantic blue crabs (Callinectes sapidus)

Gálvez, Ricardo; Guardiola Abellán, Francisco Antonio

Publication:
Evaluation of immune-related parameters in acellular and cellular fractions in male and female of Atlantic blue crabs (Callinectes sapidus)

dc.contributor.author	Gálvez, Ricardo
dc.contributor.author	Guardiola Abellán, Francisco Antonio
dc.contributor.department	Biología Celular e Histología
dc.contributor.other	Facultad de Biología
dc.date.accessioned	2026-01-29T08:49:06Z
dc.date.available	2026-01-29T08:49:06Z
dc.date.copyright	© 2025 The Authors
dc.date.issued	2025-05-25
dc.description.abstract	Brachyurans are relatively understudied compared to other decapods, with numerous fields remaining to be explored, including the immune response. Therefore, the aim of this study was to characterize several immune-related enzymes and activities of the Atlantic blue crab (Callinectes sapidus) in cell-free haemolymph (CFH) and in the haemocyte lysate supernatant (HLS), considering the gender factor and the suitability of measuring each activity in CFH or HLS. Data were expressed by millilitres of haemolymph or milligrams of protein to identify which of these units would be more robust and informative. For this, haemolymph of each gender was collected, and centrifuged to obtain CFH samples, whilst the cells were sonicated to get the HLS. Our results revealed that the protein concentration was higher in CFH compared to HLS. Considering milligrams of protein as units, phenoloxidase and esterase activities were lower in CFH regarding to HLS in both genders. Similarly, the lysozyme and phosphatase activities were lower in CFH compared to HLS but only in females. The values of lysozyme activity were higher in HLS of females compared to males. Studying millilitre of haemolymph as units, lysozyme activity exhibited higher activity in CFH compared to HLS in both genders, whilst phenoloxidase and esterase activities were higher in HLS, showing variations only in males. Interestingly, alkaline phosphatase activity was undetected in CFH male. Any variation was observed between the activities measured in both genders. Protease and antiprotease activities were higher and lower in CFH compared to HLS values, respectively, whilst any variation was observed between genders. Bactericidal activity against V. harveyi, V. alginolyticus and V. anguillarum exhibited stronger values in HLS than in CFH, independently of gender, as well as against P. damselae in females. Considering gender factor, the bactericidal activity against V. alginolyticus, V. anguillarum and P. damselae was higher in HLS of females than in males. However, the bactericidal activity against V. alginolyticus and P. damselae was higher in CFH of males compared to females. Our results could contribute to gender selection in future brachyurans studies considering the variability between males and females due to their distinct biology. Additionally, we provide data regarding the baseline values of several immune-related activities, establishing the most appropriate units from the biological point of view.
dc.format	application/pdf
dc.format.extent	10
dc.identifier.citation	Fish and Shell sh Immunology 164 (2025) 110456
dc.identifier.issn	1050-4648
dc.identifier.uri	http://hdl.handle.net/10201/196429
dc.language	eng
dc.publisher	Elsevier
dc.relation	This research forms part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17. I1) and by the Comunidad Autónoma de la Región de Murcia- Fundación Séneca (Spain). R.G. has a research contract with the University of Murcia within the “Investigo Program” in the framework of the “Recovery, Transformation and Resilience Plan”, financed by the European Union-Next Generation.
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S1050464825003456?via%3Dihub
dc.rights	Attribution 4.0 International	*
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Immune-related enzymes
dc.subject	Humoral immune response
dc.subject	Bactericidal activity
dc.subject	Atlantic blue crab (Callinectes sapidus)
dc.subject	Brachyurans
dc.subject	Haemolymph
dc.subject.ods	No relacionado con ningún objetivo de desarrollo sostenible
dc.title	Evaluation of immune-related parameters in acellular and cellular fractions in male and female of Atlantic blue crabs (Callinectes sapidus)
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication	es
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