Publication: Immunolocalization of interleukin-1 receptor antagonist in healthy and infarcted myocardium
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Date
2008
Authors
Bonetti, A. ; Marchini, M. ; Ortolani, F.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Ischemic heart disease is a widespread cause
of death. During infarction, myocardial injury is
mediated by release of several pro-inflammatory
cytokines including multifunctional interleukin-1 (IL-1).
In various tissues, IL-1-mediated deleterious effects are
known to be attenuated via the over-expression of
natural anti-inflammatory cytokine IL-1 receptor
antagonist (IL-1ra). In the present investigation, IL-1ra
distribution in healthy and infarcted myocardium was
studied by light and electron microscopy. After
immunostaining, weak positivity resulted for
cardiomyocytes in normal myocardium and, at higher
degrees, in infarction border areas and ischemic ones. In
ischemic areas, additional reactivity was displayed by
the extracellular matrix and intravascular plasma.
Immunogold labelling provided further details on
intracytoplasmatic and extracellular distribution; in
particular, noticeable gold particle distribution appeared
on intercalated discs in normal and hypertrophic
cardiomyocytes, as well as on thickened Z-lines for
these latter. The present results suggest that
cardiomyocytes represent a major source of IL-1ra in
vivo, even though additional contribution by blood
derived IL-1ra is to be taken in account in ischemic
areas. In addition, ischemia-associated intracytoplasmic IL-1ra increase and its additional presence in the
extracellular matrix is consistent with the concept that
this cytokine plays a cardioprotective role at different
levels and by distinct mechanisms.
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