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Circ_0008234 regulates the biological process of gallbladder carcinoma by targeting the miR-204-5p/FGFR2 axis

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dc.contributor.authorZhang, Na
dc.contributor.authorLi, Jing
dc.contributor.authorSun, Huapeng
dc.contributor.authorTian, Aixia
dc.contributor.authorChen, Yuhua
dc.date.accessioned2023-09-07T10:51:08Z
dc.date.available2023-09-07T10:51:08Z
dc.date.issued2023
dc.description.abstractBackground. Gallbladder carcinoma (GBC) is a common cancer disease with high mortality. Circular RNA_0008234 (circ_0008234) has been shown to play a key role in many tumors, including GBC. However, the function between circ_0008234 and microRNA-204-5p (miR-204-5p) in the progression of GBC has not been clarified. Methods. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expressions of circ_0008234, miR-204-5p and fibroblast growth factor receptor-2 (FGFR2) in GBC cells and tissues. Western blot was used to detect the expression of relative proteins. Cell proliferation, apoptosis, invasion and migration were detected by 3-(4, 5-dimethylthiazol2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) assay, flow cytometry, transwell assay and wound healing assay. Mechanically, the interaction of miR-2045p with circ_0008234/FGFR2 was notarized by dualluciferase reporter assay. A xenotransplantation model was established to study the role of circ_0008234 in vivo. Results. Circ_0008234 and FGFR2 were highly expressed in GBC tissues and cells. Silencing circ_0008234 down-regulated cell proliferation, migration and invasion of NOZ and SGC-996 cells, while miR-204-5p inhibitors reversed these effects. In addition, overexpression of FGFR2 restored the cell malignant behavior of GBC cells inhibited by miR-2045p mimic. Animal experiments confirmed the anti-tumor effect of silenced circ_0008234 in vivo. Conclusion: Circ_0008234 mediated GBC via the miR-204-5p/FGFR2 axis, providing a novel targeted therapy for gallbladder carcinomaes
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dc.format.extent13es
dc.identifier.citationHistology and Histopathology Vol. 38, nº8 (2023)
dc.identifier.doihttps://doi.org/10.14670/HH-18-538
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/133712
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCirc_0008234es
dc.subjectmiR-204-5pes
dc.subjectFGFR2es
dc.subjectGallbladder carcinomaes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleCirc_0008234 regulates the biological process of gallbladder carcinoma by targeting the miR-204-5p/FGFR2 axises
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.38, nº8 (2023)