Publication: Lectin binding in the human endometrium in early luteal phase following controlled ovarian hyperstimulation
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Date
1998
Authors
Gheri, G. ; Gheri Bryk, S. ; Taddei, G. ; Borri, P. ; Noci, I.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
A lectin histochemical study was performed
to investigate the glycoconjugate saccharidic moieties on
the endometrial epithelium and stroma in 12 women
undergoing controlled ovarian hyperstimulation (COH)
for in-vitro fertilisation for embryo transfer (IVF-ET)
in early luteal phase. 7 control subjects were also
evaluated. For this purpose a battery of seven horseradish
peroxidase-conjugated lectins (PNA, SBA, DBA,
WGA, ConA, LTA and UEA I) was used. Cytochemical
controls were performed for specificity of lectin-sugar
reaction. As far as the endometrial glands and stroma are
concerned, the obtained data showed no differences in
the endometrial lectin binding between the subjects of
the control group and the ones undergoing COH, with
the exception of PNA reactivity at the level of the apical
portion of the glandular cells, which was detected only
in COH women. It is noteworthy that, although the
endometrial dating using the Noyes's criteria showed
marked dissynchronies between the stroma and the
glands in COH subjects, a uniformity of lectin binding,
revealing the same type and localization of terminal
oligosaccharides, was observed in all the examined
subjects. The uniformity in distribution of the sugar
residues detected in the endometrial specimens
following COH might be due to the massive FSH and/or
hCG treatment which probably determines an endometrial
environment almost equal in all the examined
subjects. In all the treated subjects reactivity with
sialidase-WGA and ConA, revealing the presence of Nacetyl
-D-glucosamine and D-mannose respectively, was
detected at the level of the lining epithelium.
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