Publication: Tricalbin-Mediated contact sites control ER curvature to maintain plasma membrane integrity
Authors
Collado, Javier ; Kalemanov, Maria ; Campelo, Felix ; Bourgoint, Clelia ; Thomas, Ffion ; Loewith, Robbie ; Martínez Sánchez, Antonio ; Baumeister, Wolgang ; Stefan, Christopher J. ; Fernandez-Busnadiego, Ruben
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.devcel.2019.10.018
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info:eu-repo/semantics/article
Description
© 2019, The Author(s). This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. This document is the Submitted version of a Published Work that appeared in final form in Developmental Cell. To access the final edited and published work see https://doi.org/10.1016/j.devcel.2019.10.018
Abstract
Membrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) play fundamental roles in all eukaryotic cells. ER-PM MCS are particularly abundant in Saccharomyces cerevisiae, where approximately half of the PM surface is covered by cortical ER (cER). Several proteins, including Ist2, Scs2/22, and Tcb1/2/3 are implicated in cER formation, but the specific roles of these molecules are poorly understood. Here, we use cryo-electron tomography to show that ER-PM tethers are key determinants of cER morphology. Notably, Tcb proteins (tricalbins) form peaks of extreme curvature on the cER membrane facing the PM. Combined modeling and functional assays suggest that Tcb-mediated cER peaks facilitate the transport of lipids between the cER and the PM, which is necessary to maintain PM integrity under heat stress. ER peaks were also present at other MCS, implying that membrane curvature enforcement may be a widespread mechanism to regulate MCS function.
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Citation
Developmental Cell, Volume 51, Issue 4, 476 - 487.e7
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