Publication: Histochemical and chronological analysis of mouse submandibular gland
parenchyma subjected to abrupt reperfusion
Authors
Fujisawa, Y. ; Aiyama, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We examined the effects of abrupt
reperfusion on the mouse submandibular gland
parenchyma and determined the degree of recovery from
tissue damage. A main trophic artery supplying the
gland was ligated with silk thread, and the ligature was
then released after a variable period. The gland was
removed at various times after reperfusion and then
examined immunohistochemically and ultrastructurally.
With reperfusion after 15 or 30 min of ligation, the
tissue damage to the glands was slight or inapparent.
With reperfusion after 1 or 3 h of ligation, collapse of
the acini and the ducts was observed in parts of the
lobules, but restoration of the parenchymal structures
occurred, with the appearance of PCNA-positive cells,
although there were differences in the level of
restoration. After 6 h of ligation, most of the normal
parenchymal cells had disappeared by the 5th and 7th
days after reperfusion, and apoptosis and necrosis were
present. These findings suggest that if interruption of the
blood supply to the submandibular gland parenchyma is
limited to within a few hours, then tissue repair after
reperfusion is possible, although this will differ
according to the level of damage, because the acini and
the ducts reappear, probably with proliferation of
parenchymal cells.
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