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Retinal ganglion cell death after acute retinal ischemia is an ongoing process whose severity and duration depends on the duration of the insult

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dc.contributor.authorLafuente, M.P.
dc.contributor.authorVillegas-Pérez, M.P.
dc.contributor.authorSellés-Navarro, I.
dc.contributor.authorMayor-Torroglosa, S.
dc.contributor.authorMiralles de Imperial, J.
dc.contributor.authorVidal-Sanz, M.
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.date.accessioned2025-02-03T07:10:23Z
dc.date.available2025-02-03T07:10:23Z
dc.date.issued2002-01-18
dc.description© 2002 IBRO This document is the published version of a published work that appeared in final form in Neuroscience To access the final edited and published work see: https://doi.org/10.1016/S0306-4522(01)00458-4
dc.description.abstractIn adult Sprague–Dawley rats we have investigated retinal ganglion cell survival after transient intervals of retinal ischemia of 30, 45, 60, 90 or 120 min duration, induced by ligature of the ophthalmic vessels. Animals were killed 5, 7, 14, 21, 30, 60, 90 or 180 days later and densities of surviving retinal ganglion cells were estimated in retinal whole mounts by counting cells labelled with diAsp. This dye was applied, 3 days prior to death, to the ocular stump of the intraorbitally transected optic nerve. We found that retinal ganglion cell loss after retinal ischemia proceeds for different lengths of time. All the ischemic intervals induced loss of retinal ganglion cells whose severity and duration was related to the length of the ischemic interval. Following 30 or 45 min of ischemia, cell loss lasted 14 days and caused the death of 46 or 50%, respectively, of the population of retinal ganglion cells. Sixty, 90 or 120 min of retinal ischemia were followed by a period of cell loss that lasted up to 90 days and caused the death of 75%, 87% or 99%, respectively, of the population of retinal ganglion cells. We conclude that retinal ganglion cell loss after retinal ischemia is an ongoing process that may last up to 3 months after the injury and that its severity and duration are determined by the ischemic interval.es
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dc.identifier.citationNeuroscience. 2002 109(1):157-68.
dc.identifier.doihttps://doi.org/10.1016/S0306-4522(01)00458-4
dc.identifier.urihttp://hdl.handle.net/10201/149967
dc.languageenges
dc.publisherElsevier
dc.relationRegional Government of Murcia (Fundación Séneca, PB/18/FS/97), The Spanish Ministry of Health (FIS 98/0341; 99/0190) and The European Union (QLK6-CT-2000-00569). Tes
dc.relation.publisherversionhttps://www.ibroneuroscience.org/article/S0306-4522(01)00458-4/fulltextes
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAdult rat retinaes
dc.subjectDiAsp
dc.subjectNeuronal degeneration
dc.subjectRetinal ganglion cell death
dc.subjectTransient ischemia
dc.subjectLigature of the ophthalmic vessels
dc.titleRetinal ganglion cell death after acute retinal ischemia is an ongoing process whose severity and duration depends on the duration of the insultes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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