Publication: Angiogenesis after sintered bone implantation in rat parietal bone
Authors
Ohtsubo, S. ; Matsuda, Mikio ; Takekawa, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We studied the effect of bone substitutes on
revascularization and the restart of blood supply after
sintered bone implantation in comparison with synthetic
hydroxyapatite implantation and fresh autogenous bone
transplantation (control) in rat parietal bones. Methods
for the study included the microvascular corrosion cast
method and immunohistochemical techniques were also
used.
The revascularization of the control group was the
same as that for usual wound healing in the observations
of the microvascular corrosion casts. The sintered bone
implantation group was quite similar to that of the
control group. In the synthetic hydroxyapatite group,
immature newly-formed blood vessels existed even on
the 21st day after implantation and the physiological
process of angiogenesis was interrupted.
Immunohistochemically, vascular endothelial growth
factor (VEGF), which activates angiogenesis, appeared
at the early stages of both the control group and the
sintered bone implantation group. VEGF reduced
parallel with the appearance of the transforming growth
factor factor-beta-1 (TGF-beta-1), which obstructs
angiogenesis, and the angiogenesis passed gradually into
the mature stage. In the hydroxyapatite implantation
group, TGF-beta-1 appeared at the early stage of the
implants. The appearance of VEGF lagged and it existed
around the pores of hydroxyapatite even on the 21st day
of the implantation. Proliferation and wandering of
endothelial cells continued without any maturing of the
vessels.
These findings suggest that the structure and the
components of the implant material affect angiogenesis
after implantation as well as new bone formation.
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