Publication: Immunohistochemical identification of intracytoplasmic lumens by cytokeratin typing may differentiate renal
oncocytomas from chromophobe renal cell carcinomas
Authors
Kuroda, Naoto ; Toi, M. ; Yamamoto, M. ; Miyazaki, E. ; Hayashi, Yoshihiro ; Hiroi, Makoto ; Shuin, T. ; Enzan, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Renal oncocytomas and chromophobe renal
cell carcinomas (RCCs) share a common phenotype and
both originate from the intercalated cells of the
collecting duct. This makes it very difficult to
differentiate between the two tumors
immunohistochemically. Therefore, we studied the
results of immunohistochemistry focusing on certain
characteristic structures that are occasionally present in
renal oncocytomas. We carried out Hale’s colloidal iron
staining and immunohistochemistry for various
cytokeratins (cytokeratins 7, 8, 10, 10/13, 14, 18, 19 and
20, and AE1/AE3) in four oncocytomas and six
chromophobe RCCs. In addition, one renal oncocytoma
and one chromophobe RCC were studied using electron
microscopy. Two renal oncocytomas and one
chromophobe RCC were completely unstained by
colloidal iron. There was no evident difference between
the immunohistochemical characteristics of
oncocytomas and those of chromophobe RCCs.
However, in all four renal oncocytomas we identified
intracytoplasmic ring-like positive reactions for some
cytokeratins (at least 3 antigens of cytokeratins 7, 8 and 19, and AE1/AE3), which corresponded ultrastructurally
to the intracytoplasmic lumens (ICLs). In contrast, no
such structures were found in any of the chromophobe
RCCs using the antibodies employed. Therefore,
immunohistochemical identification of ICLs by
cytokeratin typing may be useful for differentiating
between renal oncocytomas and chromophobe RCCs and
be more sensitive in this respect than colloidal iron
staining.
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