Publication: PDCD4
regulates apoptosis in human
peritoneal mesothelial cells and promotes
gastric cancer peritoneal metastasis
Authors
Wu, Pei ; Wang, Jinou ; Mao, Xiaoyun ; Xu, Huimian ; Zhu, Zhi
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-305
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info:eu-repo/semantics/article
Description
Abstract
Objective. Programmed cell death 4
(PDCD4) is a tumor suppressor gene, however, the
function and regulatory mechanism remain to be
discovered. The connection between tumorigenesis and
apoptosis is one of the most important foci of cancer
research. Our study aimed to explore the connections
between PDCD4-mediated apoptosis of human
peritoneal mesothelial cells (HPMC) and peritoneal
metastasis in gastric cancer.
Methods. The PDCD4 expression in 31 pairs of
HPMC and tumor tissues was assessed by immunohistochemistry and RT-PCR. In cell experiments, we
monitored gastric cancer cell migration with a Transwell
chamber assay when PDCD4 was silenced in HPMC.
Subsequently, apoptosis of HPMC was detected by a
flow cytometric assay and western blotting. After
analyzing cytokines in culture supernatants from gastric
cancer with enzyme-linked immunosorbent assays
(ELISAs), transforming growth factor-beta 1 (TGF-β1)
was abundant in the culture supernatants of gastric
cancer. Then, PDCD4 expression in HMrSV5 cells was
analyzed by western blotting after retreatment with
different concentrations of TGF-β1. Moreover, apoptosis
of peritoneal mesothelial cells treated with TGF-β1 was
detected according to the above methods.
Results. In human metastatic peritoneal tissues, the
expression of PDCD4 was significantly lower than that
in normal tissues. At the same time, decreased
expression of PDCD4 in HPMC was associated with
increased migration capacity of gastric cancer cells.
Moreover, suppressing the expression of PDCD4
promoted apoptosis in mesothelial cells which may be
regulated by TGF-β secreted from gastric cancer cells.
Conclusions. These data suggested that decreased
expression of PDCD4 significantly promoted apoptosis
in human peritoneal mesothelial cells, thus inducing
peritoneal metastasis, and that TGF-β1 secreted from
gastric cancer cells may have played a crucial role.
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Citation
Histology and Histopathology Vol. 36, nº4 (2021)
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Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/