Publication: Dark-cell areas in the dog vestibular endorgans: an immunohistochemical study
Authors
Coppens, A.G. ; Salmon, I. ; Heizman, C.W. ; Poncelet, L.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The stria vascularis in the cochlea and the
dark-cell areas in the vestibular endorgans are structures
involved in the production of endolymphatic fluid. This
study investigated the dark-cell areas in the vestibular
endorgans of the dog by classical staining and by
immunohistochemistry (anti-Na,K-ATPase ß2 isoform,
anti-cytokeratins (against cytokeratins 5 and 8), antivimentin
and anti-S100A6) from birth to 110 postnatal
days.
Using classical staining, it was not possible to
discriminate dark cells from other epithelial cells lining
the vestibular endolymphatic spaces. From birth, the
Na,K-ATPase ß2 isoform was expressed in the lateral
and basal cell membranes of a subset of cells located in
the utricular wall, at the base of the cristae ampullaris
and was identified as dark cells. From birth, anticytokeratins
labelled all the cells forming the epithelial
lining, including the dark cells, while anti-vimentin
labelled the underlying mesenchymal cells. From
postnatal day 10, anti-S100A6 labelled subepithelial
cells exclusively located underneath the dark-cell areas
and were identified as vestibular melanocyte-like cells.
From birth, Fontana staining evidenced fine melanin
granules in the subepithelial layer. The amount of
melanin granules increased during the first month.
Melanin distribution was closely associated with the
region where S100A6-positive cells were located.
The cell-specific antigen expression in the dog darkcell
areas was clearly comparable to that of the dog stria
vascularis previously described. The present
investigation also suggested an earlier histological and
immunohistological maturity in the dark-cell areas than
in the stria vascularis of dogs.
This preliminary morphological description of the
normal dark-cell areas in dogs by means of immunomarkers may be instrumental in studying
pathological processes involving the fluid-secreting
structures in vestibular endorgans.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.