Publication: Histochemical study of apoptotic epithelial cells depending on testosterone in primary cultured rat prostatic tissues
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Date
2000
Authors
Furuya, T. ; Kubo, M. ; Ueno, A. ; Fuji, Y. ; Baba, T. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
To clarify whether apoptosis can be induced
in cultured rat prostatic epithelial cells, they were
investigated at various time points, depending on
different concentrations of testosterone. Ventral lobes of
rat prostates were cultured as small pieces of tissues up
to 14 days. They were examined by anti-Fas antibody
immunostaining and also compared to findings revealed
by in situ end-labelling (ISEL) technique. To clarify
apoptotic nuclei at high resolution, the quick-freezing
and deep-etching (QF-DE) method was also used, as
reported before. The localization and appearance of Faspositive
cells were detected more widely and earlier than
those of ISEL-positive cells, but both label-positive
localizations were closely related to each other. In
addition, they were detected more often in epithelial
cells cultured with low testosterone concentrations. By
the QF-DE method, chromatin fibers were found to be
broken in spotty parts of apoptotic nuclei. We could
control the concentration of testosterone in culture
medium and detect the appearance of Fas antigen in
cultured prostatic epithelial cells, followed by apoptotic
changes. So, Fas and Fas-ligand system is one candidate
for apoptosis in the prostate glands, depending on
removal of hormonal testosterone.
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