Effects of water salinity on melatonin levels in plasma and peripheral tissues and on melatonin binding sites in European sea bass (Dicentrarchus labrax)

López Olmeda, José Fernando; Oliveira, Catarina; Kalamarz, Hanna; Kulczykowska, Ewa; Delgado, María Jesús; Sánchez Vázquez, Francisco Javier

Publication:
Effects of water salinity on melatonin levels in plasma and peripheral tissues and on melatonin binding sites in European sea bass (Dicentrarchus labrax)

dc.contributor.author	López Olmeda, José Fernando
dc.contributor.author	Oliveira, Catarina
dc.contributor.author	Kalamarz, Hanna
dc.contributor.author	Kulczykowska, Ewa
dc.contributor.author	Delgado, María Jesús
dc.contributor.author	Sánchez Vázquez, Francisco Javier
dc.contributor.department	Fisiología
dc.date.accessioned	2024-02-09T11:26:56Z
dc.date.available	2024-02-09T11:26:56Z
dc.date.issued	2009
dc.description	©<2009>. This manuscript version is made available under the CC-BY-NC-ND license http://creativecommons.org/licenses/ccby-nc-nd/4.0/ This document is the Acepted version of a Published Work that appeared in final form in [Comparative Biochemistry and Physiology]. To access the final edited and published work see [https://doi.org/10.1016/j.cbpa.2008.12.003]
dc.description.abstract	Sea bass is a euryhaline fish that lives in a wide range of salinities and migrates seasonally from lagoons to the open sea. However, to date, the influence of water salinity on sea bass melatonin levels has not been reported. Here, we evaluated the differences in plasma and tissue melatonin contents and melatonin binding sites in sea bass under four different salinities: seawater (36 ‰), isotonic water (15 ‰), brackish (4 ‰) and freshwater (0 ‰). Melatonin content was evaluated in plasma, whole brain, gills, intestine and kidney, while melatonin binding sites were analyzed in different brain regions and in the neural retina. Plasma melatonin levels at mid-dark varied among salinities, with the lowest value occurring at seawater salinity (102 pg/ml), and the highest at freshwater (151 pg/ml). In gills and intestine, however, the highest melatonin values were found in the seawater group (209 and 627 pg/g tissue, respectively). Melatonin binding sites in the brain also varied with salinity, with the highest density being observed at the lower salinities in optic tectum, cerebellum and hypothalamus (30.3, 13.0, and 8.0 fmol/mg protein, respectively). Melatonin binding sites in the retina showed a similar pattern, with the highest values in the fish maintained in freshwater. Taken together, these results revealed that salinity influences melatonin production and modifies the density of binding sites, which would point to a role for this hormone in timing seasonal events in sea bass, including those linked to fish migration between waters of different salinities.
dc.format	application/pdf	es
dc.format.extent	17
dc.identifier.citation	Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 2009 Apr;152(4):486-90
dc.identifier.doi	https://doi.org/10.1016/j.cbpa.2008.12.003
dc.identifier.issn	Print: 1095-6433
dc.identifier.issn	Electrónico: 1531-4332
dc.identifier.uri	http://hdl.handle.net/10201/139094
dc.language	eng	es
dc.publisher	Elsevier
dc.relation	This study was supported by the Spanish Ministry of Science and Technology (MCYT) by projects AGL2004-07984-C02-01 granted to FJSV and AGL2004-08137-C04-01 granted to MJD; and by a travel grant from the University of Murcia granted to JFLO.	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Melatonin
dc.subject	Melatonin binding sites
dc.subject	Dicentrarchus labrax
dc.subject	Seasonality
dc.subject	Salinity
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Ciencias biológicas en general
dc.title	Effects of water salinity on melatonin levels in plasma and peripheral tissues and on melatonin binding sites in European sea bass (Dicentrarchus labrax)	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es