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Considerations about the inhibition of monophenolase and diphenolase activities of tyrosinase. Characterization of the inhibitor concentration which generates 50 % of inhibition, type and inhibition constants. A review

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dc.contributor.advisorSaura Sanmartín, Adrián
dc.contributor.advisorGarcía Molina, Francisco
dc.contributor.authorGarcía Molina, Pablo
dc.contributor.authorSaura Sanmartín, Adrián
dc.contributor.authorBerná Cánovas, José
dc.contributor.authorMuñoz Muñoz, Jose Luis
dc.contributor.authorGarcía Cánovas, Francisco
dc.contributor.authorGarcía Molina, Francisco
dc.contributor.authorRodríguez López, José Neptuno
dc.contributor.authorTeruel Puche, José Antonio
dc.contributor.departmentQuímica Orgánica
dc.date.accessioned2024-04-17T10:14:30Z
dc.date.available2024-04-17T10:14:30Z
dc.date.issued2024-04-10
dc.description©2024 The Authors. This manuscript version is made available under the CC-BY-NC 4.0 license http://creativecommons.org/licenses/by-nc/4.0/. This document is the Published version of a Published Work that appeared in final form in International Journal of Biological Macromolecules. To access the final edited and published work see https://doi.org/10.1016/j.ijbiomac.2024.131513es
dc.description.abstractTyrosinase is a copper oxidase enzyme which catalyzes the first two steps in the melanogenesis pathway, L tyrosine to L-dopa conversion and, then, to o-dopaquinone and dopachrome. Hypopigmentation and, above all, hyperpigmentation issues can be originated depending on their activity. This enzyme also promotes the browning of fruits and vegetables. Therefore, control of their activity by regulators is research topic of great relevance. In this work, we consider the use of inhibitors of monophenolase and diphenolase activities of the enzyme in order to accomplish such control. An experimental design and data analysis which allow the accurate calculation of the degree of inhibition of monophenolase activity (iM) and diphenolase activity (iD) are proposed. The IC50 values (amount of inhibitor that causes 50 % inhibition at a fixed substrate concentration) can be calculated for the two activities and from the values of ICM 50 (monophenolase) and ICD 50(diphenolase). Addi tionally, the strength and type of inhibition can be deduced from these values. The data analysis from these ICD 50 values allows to obtain the values of Kapp I1 or Kapp I2 , or Kapp I1 and Kapp I3 from the values of ICM 50. In all cases, the values of the different Kapp I must satisfy their relationship with ICM 50 and ICD 50.
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.citationInternational Journal of Biological Macromolecules, 267 (2024) 131513
dc.identifier.doihttps://doi.org/10.1016/j.ijbiomac.2024.131513
dc.identifier.issnPrint: 0141-8130
dc.identifier.urihttp://hdl.handle.net/10201/140941
dc.languageenges
dc.publisherElsevieres
dc.relationFundacion Séneca, the Region de Murcia (FS-RM) (20809/PI/18). Northumbria University (internal grant). European Union-NextGenerationEU (Margarita Salas postdoctoral contract, Ministerio de Universidades of the Spanish Government).es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución-NoComercial 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectTyrosinasees
dc.subjectMonophenolase activityes
dc.subjectDiphenolase activityes
dc.subjectInhibitiones
dc.subjectDegree of inhibitiones
dc.subjectMonophenolase activity
dc.subjectIC50
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleConsiderations about the inhibition of monophenolase and diphenolase activities of tyrosinase. Characterization of the inhibitor concentration which generates 50 % of inhibition, type and inhibition constants. A reviewes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication36d7ac7e-b9a7-477c-8f7e-f03d4cc834c1
relation.isAuthorOfPublicationbd0ae640-4c40-439e-bf79-a87fea6c8898
relation.isAuthorOfPublication.latestForDiscovery36d7ac7e-b9a7-477c-8f7e-f03d4cc834c1
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