Inhibition of sarcoplasmic reticulum Ca2+-ATPase by miconazole

Lax Pérez, Antonio Manuel; Fernández Belda, Francisco; Soler Pardo, Fernando

Publication:
Inhibition of sarcoplasmic reticulum Ca2+-ATPase by miconazole

dc.contributor.author	Lax Pérez, Antonio Manuel
dc.contributor.author	Fernández Belda, Francisco
dc.contributor.author	Soler Pardo, Fernando
dc.contributor.department	Medicina
dc.date.accessioned	2024-01-23T09:17:37Z
dc.date.available	2024-01-23T09:17:37Z
dc.date.issued	2002-07
dc.description	©2002. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in American Journal of Physiology: Cell Physiology. To access the final edited and published work see https://doi.org/10.1152/ajpcell.00580.2001	es
dc.description.abstract	The inhibition of sarcoplasmic reticulum Ca2+-ATPase activity by miconazole was dependent on the concentration of ATP and membrane protein. Half-maximal inhibition was observed at 12 microM miconazole when the ATP concentration was 50 microM and the membrane protein was 0.05 mg/ml. When ATP was 1 mM, a low micromolar concentration of miconazole activated the enzyme, whereas higher concentrations inhibited it. A qualitatively similar response was observed when Ca2+ transport was measured. Likewise, the half-maximal inhibition value was higher when the membrane concentration was raised. Phosphorylation studies carried out after sample preequilibration in different experimental settings shed light on key partial reactions such as Ca2+ binding and ATP phosphorylation. The miconazole effect on Ca2+-ATPase activity can be attributed to stabilization of the Ca2+-free enzyme conformation giving rise to a decrease in the rate of the Ca2+ binding transition. The phosphoryl transfer reaction was not affected by miconazole.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.citation	American Journal of Physiology: Cell Physiology. 2002 Jul 283(1):C85-92.doi: 10.1152/ajpcell.00580.2001.
dc.identifier.doi	10.1152/ajpcell.00580.2001
dc.identifier.issn	1522-1563
dc.identifier.issn	0363-6143
dc.identifier.uri	http://hdl.handle.net/10201/137582
dc.language	eng	es
dc.relation	This study was funded by grants PB97-1039 from the Spanish Ministerio de Ciencia y Tecnologia and PI-22/00756/FS/01 from Fundacion Seneca de la Region de Murcia, Spain.	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.title	Inhibition of sarcoplasmic reticulum Ca2+-ATPase by miconazole	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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relation.isAuthorOfPublication.latestForDiscovery	fb419085-b327-4302-860b-d0f7e5000ea9