Publication: lmmunohistochemical expression of Bcl-2 oncoprotein in EBV-associated nasopharyngeal carcinoma correlated to histological type and survival
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Date
1997
Authors
vera-Sempere, F.J. ; Burgos, J.S. ; Botella, M.S. ; Morera, C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Expression of Bcl-2 is associated with
inhibition of apoptosis and extension of cell survival. In
vitro Bcl-2 protein expression is up-regulated by the
EBV-latency associated antigen latent membrane protein
(LMP-1). We have investigated the relationship between
the presence of EBV-DNA screened by means of
sensitive nested-PCR, nasopharyngeal carcinoma (NPC)
histological types according to two different schemata
(WHO and Micheau classifications) and Bcl-2-124
immunohistochemical expression in 55 biopsy samples
of NPC. EBV genome was detected in 100% of samples
with sufficient DNA quality to support the previous view
that al1 types of NPC are variants of EBV-infected
neoplasia. Bcl-2 was observed in the basa1 layer of
normal nasopharyngeal mucosa and also at cytoplasmic
level in 42 of 55 (76.4%) NPC cases. Mitotic
neoplastic cells usually showed strong cytoplasmic and
chromosomal staining, a finding not well referred to
previously. Bcl-2 expression was significantly associated
(p<0.05) to undifferentiated NPC (UNPC) when a
histological classification with only two major
microscopical types was applied. No close correlations
were found between the presence of EBV-DNA, NPC
location, clinical stage and age or sex of the patients in relation to Bcl-2 positive expression. However, when
comparing Bcl-2 expression and known survival mean
of the patients, significant differences were observed
(p<0.001) so that mean survivals were 3 1.1, 24.4, 52.2
and 54.1 months respectively for NPC patients with -, +,
++ and +++ Bcl-2 immunoreactivity. Nevertheless this
better clinical outcome in Bcl-2 NPC positive cases may
depend on the histological type due to close relationship
with UNPC. Only studies of larger series with long-term
follow-up and multivariate analyses may document
whether Bcl-2 expression is an independent prognostic
marker in the evolution of NPC patients.
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