Publication: The spectrum of cytokeratins expressed in the
adult human cornea, limbus and perilimbal conjunctiva
Authors
Merjava, Stanislava ; Neuwirth, Ales ; Tanzerova, Michaela ; Jirsova, Katerina
item.page.secondaryauthor
item.page.director
Publisher
Murcia: F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The aim of this study was to detect a
spectrum of cytokeratins (CK) present in the adult
human cornea, limbus and perilimbal conjunctiva.
Cryosections from seven corneo-scleral discs were
fixed, and indirect immunofluorescent staining was
performed using antibodies directed against CK1-CK10
and CK13-CK20. The percentage of positive cells was
calculated in the epithelium of the cornea, limbus and
perilimbal conjunctiva. Quantitative real time RT-PCR
(qRT-PCR) was used to detect CK6 and CK18
expression in the corneal and conjunctival epithelium.
The most intense staining present throughout the
cornea was observed for CK3, CK5 and CK14; CK19
was found at the corneal periphery only. CK4 and
CK10/13 revealed mild to moderate positivity mostly in
the superficial layers of the cornea. The suprabasal cell
layers of all examined areas showed a strong positivity
for CK16. A heterogeneous staining pattern with a
centrifugal decrease in the signal was observed for CK8
and CK18. CK5/6, CK14 and CK19 were present in the
limbus, where a positive signal for CK3 was observed in
the suprabasal and superficial cells only. In contrast to
the cornea, CK15 appeared in the basal and suprabasal
layers of the limbus. The perilimbal conjunctiva showed
strong immunostaining for CK10/13, CK14 and CK19.
A moderate signal for CK7 was detected in the
superficial layers of the conjunctiva. qRT-PCR
confirmed CK6 and CK18 expression in the corneal and
conjunctival epithelium.
The detailed characterization of the corneal, limbal
and perilimbal conjunctival epithelium under normal
circumstances may be useful for characterizing the
changes occurring under pathological conditions.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.