Publication: A novel approach to the growth analysis of hamster secondary palate by histone 3 m RNA in situ hybridiration
Loading...
Date
1994
Authors
Shah, R.M. ; Young, A.V. ; Song, B.Z. ; Wong, D.T.W.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
A study was undertaken to determine the cell
proliferation kinetics during the development of hamster
vertical palatal shelf ad initium. Harnster embryo heads,
obtained at different times between days 10 and 12 of
gestation (which is the period of vertical shelf
development) were processed and sectioned to localize
histone 3 mRNA, a cell cycle specific gene, by in situ
hybridization. Sense and antisense 35~-labelledh istone 3
riboprobes were used as hybridization probes. Percent
labelled cells were determined. The results showed that a
high rate of random proliferation of both epithelial and
mesenchymal cells was a major component of early
vertical palatal growth. Subsequently, during the latter
half of vertical shelf development, the proliferation rates
of the epithelial and mesenchymal cells were different in
a region specific manner. It was suggested that the
spatio-temporal changes in the distribution of cycling
mesenchymal and epithelial cells during vertical palate
development may indicate their heterogeneity for
subsequent segregation into appropnate phenotypes.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.