Publication: lmmunohistochemical reaction of myocardial fibers with actin antiserum in autopsy cases of myocardial infarction
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Date
1986
Authors
Shigeo Hashimoto ; Shozo Nishida ; Shingo Hiruma ; Manabu Takahashi ; Yoshiki Enomoto ; Kuniyasu Sakatani
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The purpose of this study was to determine the
immunoreactivity of myocardial actin filaments with actin
antiserum and to examine the significance of its application
to diseased human cardiac muscle.
The actin was extracted and purified from chicken
gizzards. Anti-actin rabbit serum was prepared and purified
by affinity chromatography and defined by an
immunoblotting test.
Using the avidin-biotin-peroxidase complex (ABC)
method, the actin antiserum was applied to paraffin sections
prepared from hearts taken from routine autopsies of
patients who had died of myocardial infarction.
Reactivity was shown to be completely lost, not only
in necrotized fibers, but also in non-specific degenerative
fibers which could be identified by their eosinophilic
cytoplasm with pyknotic nuclei, and clearly remaining
and/or diminished cross-striations stained with
hematoxylin-eosin. In contrast, hypertrophic myocardial
fibers adjacent to granulation or scar tissue and those
adjacent to infarcted foci exhibited a more intense reaction.
These results indicated that the immunohistochemical
reaction of actin filaments can be used for the easy detection
of very mild degrees of degeneration of cardial muscle
fibers, and for hypertrophic fibers adjacent to diseased foci.
Studies of the immunoreactivity of actin protein
suggestive of alteration at the molecular level might yield
morphological clues regarding the nature of functional
activity in the contraction of cardiac fibers.
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