Consumption of spinach and tomato modifies lipid metabolism, reducing Hepatic Steatosis in rats

Elvira Torales, Laura Inés; Navarro González, Inmaculada; Rodrigo García, Joaquín; García Alonso, Javier; Periago Castón, María Jesús; Seva Alcaraz, Juan

Publication:
Consumption of spinach and tomato modifies lipid metabolism, reducing Hepatic Steatosis in rats

dc.contributor.author	Elvira Torales, Laura Inés
dc.contributor.author	Navarro González, Inmaculada
dc.contributor.author	Rodrigo García, Joaquín
dc.contributor.author	García Alonso, Javier
dc.contributor.author	Periago Castón, María Jesús
dc.contributor.author	Seva Alcaraz, Juan
dc.contributor.department	Anatomía y Anatomía Patológica Comparadas
dc.date.accessioned	2024-11-07T11:40:00Z
dc.date.available	2024-11-07T11:40:00Z
dc.date.issued	2020-10-24
dc.description	© 2020 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the Published version of a Published Work that appeared in final form in Antioxidants. To access the final edited and published work see https://doi.org/10.3390/antiox9111041	es
dc.description.abstract	Non-alcoholic fatty liver disease (NAFLD) is currently a serious and growing clinical problem in developed and developing countries and is considered one of the most frequent chronic liver diseases in the world. The aim of this study was to evaluate the functionality of dietary carotenoids provided by tomato and spinach in the dietary treatment of steatosis. Twenty-two Sprague-Dawley rats with induced steatosis were grouped into three groups and fed standard diet (CD group) and two experimental diets supplemented with 12.75% (LC12.75 group) and 25.5% (HC25.5 group) of a mixture of spinach and tomato powder. Rats fed carotenoid-rich feeds showed an improvement in the plasma biomarkers of steatosis, with lower levels of glucose, total cholesterol, VLDL, TG, proteins, ALT and AST. Likewise, a decrease in oxidative stress was observed, with a significant reduction of malondialdehyde (MDA) in plasma (up to 54%), liver (up to 51.42%) and urine (up to 78.89%) (p < 0.05) and an increase in plasma antioxidant capacity (ORAC) (up to 73.41%) (p < 0.05). Furthermore, carotenoid-rich diets led to an accumulation of carotenoids in the liver and were inversely correlated with the content of total cholesterol and hepatic triglycerides, increasing the concentrations of MUFA and PUFA (up to 32.6% and 48%, respectively) (p < 0.05). The accumulation of carotenoids in the liver caused the modulation of genes involved in lipid metabolism, and we particularly observed an overexpression of ACOX1, APOA1 and NRIH2 (LXR) and the synthesis of the proteins. This study suggests that dietary carotenoids from spinach and tomato aid in the dietary management of steatosis by reversing steatosis biomarkers.	es
dc.format	application/pdf	es
dc.format.extent	21	es
dc.identifier.citation	Antioxidants, 2020, Vol. 9, N. 11 : 1041
dc.identifier.doi	https://doi.org/10.3390/antiox9111041
dc.identifier.issn	Electronic: 2076-3921
dc.identifier.uri	http://hdl.handle.net/10201/146085
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	This work was supported by the projects MINECO (Spanish)/FEDER-EU BIO2012-38103.	es
dc.relation.publisherversion	https://www.mdpi.com/2076-3921/9/11/1041	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Spinach	es
dc.subject	Tomato	es
dc.subject	Carotenoids	es
dc.subject	Hepatic steatosis	es
dc.subject	Lipid metabolism	es
dc.subject	Gene expression	es
dc.subject	Proteomic	es
dc.title	Consumption of spinach and tomato modifies lipid metabolism, reducing Hepatic Steatosis in rats	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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