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Epididymal and ejaculated sperm functionality is regulated differently by periovulatory oviductal fluid in pigs

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dc.contributor.authorSoriano-Úbeda, Cristina
dc.contributor.authorAvilés-López, Karen
dc.contributor.authorGarcía Vázquez, Francisco Alberto
dc.contributor.authorRomero Aguirregomezcorta, Jon
dc.contributor.authorMatas Parra, Carmen
dc.contributor.departmentAnatomía y Anatomía Patológica Comparada
dc.contributor.otherFacultad de Veterinaria
dc.date.accessioned2026-01-19T12:06:41Z
dc.date.available2026-01-19T12:06:41Z
dc.date.copyright© 2020, American Society of Andrology and European Academy of Andrology
dc.date.issued2020-09-13
dc.description.abstractBackground: The current results of in vitro reproduction techniques in pigs, such as in vitro fertilization (IVF) and embryo development, show high performance with both epididymal and ejaculated spermatozoa. However, the results using ejaculated spermatozoa are even better. Ejaculated spermatozoa are exposed to the secretions of the accessory seminal glands: the seminal plasma (SP). It has been reported that exposure of spermatozoa to reproductive fluids, such as SP or periovulatory oviductal fluid (pOF), modulates sperm functionality both in vivo and in vitro. But whether or not this modulating effect of pOF depends on the origin of the spermatozoa being epididymal or ejaculated, is still unknown. Objectives: To determine and compare the effect of pOF on epididymal and ejaculated sperm functionality. Material and methods: The effects of incubating spermatozoa from the epididymis and ejaculate with pOF in capacitating conditions were investigated by analyzing sperm motility, phosphorylation of protein kinase A substrates and proteins in tyros ine (pPKAs and pTyr, respectively), the interaction of the spermatozoa with the oocyte in IVF and intracytoplasmic sperm injection (ICSI), and, finally, the spermatozoa chromatin condensation status.Results: the pOF modified events related to capacitation in epididymal spermatozoa by decreasing motility, pPKAs and pTyr. In the interaction with the oocyte after sperm capacitation, pOF regulated the epididymal and ejaculated spermatozoa differently. While pOF decreased the number of spermatozoa bound to the zona pellucida (Spz/ZP) and increased oocyte activation after ICSI with epididymal spermatozoa, with the ejaculated spermatozoa, it decreased the mean number penetrating each oocyte (Spz/O). Additionally, pOF significantly increased the nuclear decondensation of the epididymal spermatozoa after the fertilization of the oocyte.Conclusion: The modulation of sperm functionality by pOF is conditioned by the origin of the spermatozoa.
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dc.format.extent14
dc.identifier.citationAndrology. 2021;9:426–439
dc.identifier.doihttps://doi.org/10.1111/andr.12902Digital Object Identifier (DOI)
dc.identifier.eissn2047-2927
dc.identifier.issn2047-2919
dc.identifier.urihttp://hdl.handle.net/10201/188350
dc.languageeng
dc.publisherWiley
dc.relationThis work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) (grants AGL2015-66341-R and PID2019-106380RB-I00), and Fundación Séneca (grant 20040/ GERM/16).
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/andr.12902
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCapacitation
dc.subjectEpididymal spermatozoa
dc.subjectIVF
dc.subjectOviductal fluid
dc.subjectSeminal plasma
dc.subjectBoar
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleEpididymal and ejaculated sperm functionality is regulated differently by periovulatory oviductal fluid in pigs
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublication24843fe8-fa96-4462-a6c9-b3a817588cbf
relation.isAuthorOfPublication3cc83bb3-8c44-471d-bc14-7d7dff677509
relation.isAuthorOfPublicationa5bdc1c7-b6e8-4685-99cc-d8a3e9761b5f
relation.isAuthorOfPublication.latestForDiscovery24843fe8-fa96-4462-a6c9-b3a817588cbf
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