Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass

Garcia Alvarez, M. A.; Chaves Pozo, E.; Cuesta Peñafiel, Alberto

Publication:
Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass

dc.contributor.author	Garcia Alvarez, M. A.
dc.contributor.author	Chaves Pozo, E.
dc.contributor.author	Cuesta Peñafiel, Alberto
dc.contributor.department	Biología Celular e Histología
dc.date.accessioned	2024-02-14T23:11:32Z
dc.date.available	2024-02-14T23:11:32Z
dc.date.issued	2024-03
dc.description	© 2023. The authors. This document is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc /4.0/ This document is the accepted version of a published work that appeared in final form in Developmental and Comparative Immunology
dc.description.abstract	Cell-mediated cytotoxicity (CMC) is essential in eradicating virus-infected cells, involving CD8+ T lymphocytes (CTLs) and natural killer (NK) cells, through the activation of different pathways. This immune response is well-studied in mammals but scarcely in teleost fish. Our aim was to investigate the adaptive CMC using head-kidney (HK) cells from European sea bass infected at different times with nodavirus (NNV), as effector cells, and the European sea bass brain cell line (DLB-1) infected with different NNV genotypes, as target cells. Results showed low and unaltered innate cytotoxic activity through the infection time. However, adaptive CMC against RGNNV and SJNNV/RGNNV-infected target cells increased from 7 to 30 days post-infection, peaking at 15 days, demonstrating the specificity of the cytotoxic activity and suggesting the involvement of CTLs. At transcriptomic level, we observed up-regulation of genes related to T cell activation, perforin/granzyme and Fas/FasL effector pathways as well as apoptotic cell death. Further studies are necessary to understand the adaptive role of European sea bass CTLs in the elimination of NNV-infected cells.
dc.format	application/pdf	es_ES
dc.identifier.citation	Developmental & Comparative Immunology Volume 152, March 2024, 105124
dc.identifier.doi	https://doi.org/10.1016/j.dci.2023.105124
dc.identifier.issn	0145-305X
dc.identifier.uri	http://hdl.handle.net/10201/139389
dc.language	eng	es_ES
dc.publisher	Elsevier
dc.relation.isreferencedby	ED_IDENTRADA=1351
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0145305X23012776?via%3Dihub
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Cytotoxic T lymphocytes (CTLs)
dc.subject	Perforin
dc.subject	Granzyme
dc.subject	Nodavirus
dc.subject	DLB-1
dc.subject.other	European sea bass
dc.title	Cytotoxic activity and gene expression during in vitro adaptive cell-mediated cytotoxicity of head-kidney cells from betanodavirus-infected European sea bass	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dspace.entity.type	Publication	es
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