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Development of decellularization protocols for female cat reproductive organs

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dc.archivorevisadoScaffold
dc.contributor.authorSanguansook, P
dc.contributor.authorMartínez-López, Cristina
dc.contributor.authorLópez-Orozco, Marina
dc.contributor.authorChatdarong, K
dc.contributor.authorGarcía-Vázquez, FA
dc.contributor.authorMartínez Cáceres, Carlos Manuel
dc.contributor.authorIzquierdo Rico, María José
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-01-28T16:24:35Z
dc.date.available2025-01-28T16:24:35Z
dc.date.issued2024-06
dc.description© 2024 Authors This document is the published version of a published work that appeared in final form in Research in Veterinary Science This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0 To access the final edited and published work see: https://doi.org/10.1016/j.rvsc.2024.105257
dc.description.abstractDecellularization is an innovative method to create natural scaffolds by removing all cellular materials while preserving the composition and three-dimensional ultrastructure of the extracellular matrix (ECM). The obtention of decellularized reproductive organs in cats might facilitate the development of assisted reproductive techniques not only in this species but also in other felids. The aim was to compare the efficiency of three decellularization protocols on reproductive organs (ovary, oviduct, and uterine horn) in domestic cats. The decellularization protocol involved 0.1% sodium dodecyl sulfate and 1%Triton X-100. Protocol 1 (P1) entailed 2- cycles of decellularization using these detergents. Protocol 2 (P2) was like P1 but included 3-cycles. Protocol 3 (P3) was similar to P2, with the addition of deoxyribonuclease incubation. Reproductive organs from nine cats were separated into two sides. One side served as the control (non-decellularized organ) while the contralateral side was the treated group (decellularized organ). The treated organs were subdivided into 3 groups (n = 3 per group) for each protocol. Both control and treated samples were analyzed for DNA content, histology (nuclear and ECM (collagen, elastin, and glycosaminoglycans (GAGs)) density), ultrastructure by electron microscopy, and cytotoxicity. The results of the study showed that P3 was the only protocol that displayed no nucleus residue and significantly reduced DNA content in decellularized samples (in all the studied organs) compared to the control (P < 0.05). The ECM content in the ovaries remained similar across all protocols compared with controls (P > 0.05). However, elastic fibers and GAGs decreased in decellularized oviducts (P < 0.05), while collagen levels remained unchanged (P > 0.05). Regarding the uterus, the ECM content decreased in decellularized uterine horns from P3 (P < 0.05). Electron microscopy revealed that the microarchitecture of the decellularized samples was maintained compared to controls. The decellularized tissues, upon being washed for 24 h, showed cytocompatibility following co-incubation with sperm. In conclusion, when comparing different decellularization methods, P3 proved to be the most efficient in removing nuclear material from reproductive organs compared to P1 and P2. P3 demonstrated its success in decellularizing ovarian samples by significantly decreasing DNA content while maintaining ECM components and tissue microarchitecture. However, P3 was less effective in maintaining ECM contents in decellularized oviducts and uterine horns.
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dc.format.extent11es
dc.identifier.citationResearch in Veterinary Science 173 (2024) 105257
dc.identifier.doihttps://doi.org/10.1016/j.rvsc.2024.105257
dc.identifier.issnPrint.: 0034-5288
dc.identifier.issnElectronic.: 1532-2661
dc.identifier.urihttp://hdl.handle.net/10201/149478
dc.languageenges
dc.publisherElsevieres
dc.relationThis research project is supported by Second Century Fund (C2F, Batch 1, 2019), Chulalongkorn university, PID2021-123091NB-C21 financiado por MCIN/ AEI /10.13039/501100011033/ y por FEDER Una manera de hacer Europa, and by the Ministry of Science and Innovation (PID2019-106380RB-I00/AEI/10.13039/501100011033).es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0034528824001231?via%3Dihub
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBioengineering
dc.subjectDecellularization
dc.subjectFelis catus
dc.titleDevelopment of decellularization protocols for female cat reproductive organses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicationa11bfbe4-fe50-4173-a96d-aa785375a6be
relation.isAuthorOfPublicationb0205f82-a44c-47b6-8061-b55efb4e6717
relation.isAuthorOfPublication.latestForDiscoverya11bfbe4-fe50-4173-a96d-aa785375a6be
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