Publication: Sunburn reaction in the dorsal skin of hypotrichotic WBN/ILA-Ht rats
Authors
Okada, T. ; Albarenque, S.M. ; Yasoshima, A. ; Malcotti, V. ; Katayama, K. ; Uetsuka, K. ; Nakayama, Hiroyuki ; Doi, K.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The dorsal skin responses to a single
irradiation with a high-dose of UVB (10kJ/m2) were
examined histologically and immunohistochemically in
UVB-sensitive Wistar-derived hypotrichotic WBN/ILAHt
rats (HtRs). Sunburn cells (SBCs) which were
characterized by pyknotic nuclei and eosinophilic
cytoplasm and had ultrastructual characteristics of
apoptotic cells were first observed in the epidermis at 3
hours (h) after irradiation. The number peaked at 6 h,
and then decreased rapidly. The expressions of p53
protein, which is known to be closely related to the
formation of SBCs, and of p21 protein, which is one of
the transcriptional target genes of p53, were
immunohistochemically detected, and their labeling
index (LI) in the epidermis peaked at 12 to 24 h (p53) or
at 24h (p21) after irradiation. On the other hand,
proliferating cell nuclear antigen (PCNA)-LI in
keratinocytes was significantly lower than the control
group at 6 h after irradiation and thereafter it increased
and became significantly higher than the control group from 24 to 48 h. At 48 h, moderate hyperplasia with
moderate numbers of mitotic keratinocytes was first
observed in the epidermis. In the dermis, mild edema
developed from 12 to 36 h and it accompanied mild
lymphocyte infiltration at 36 h. Judging from the present
results, it was suggested that some factors other than p53
might be involved in SBC formation, and that p53 might
induce p21 protein and play an important role in cell
growth arrest in keratinocytes after UVB irradiation.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.