Morphological characterization of renal cell lines (BGM and VERO) exposed to low doses of lead nitrate.

Romero García, Diego; Gómez Zapata, Maximiliano; Luna, A.; García Fernández, Antonio Juan

Publication:
Morphological characterization of renal cell lines (BGM and VERO) exposed to low doses of lead nitrate.

dc.contributor.author	Romero García, Diego
dc.contributor.author	Gómez Zapata, Maximiliano
dc.contributor.author	Luna, A.
dc.contributor.author	García Fernández, Antonio Juan
dc.contributor.department	Ciencias Sociosanitarias
dc.date.accessioned	2024-03-12T11:33:19Z
dc.date.available	2024-03-12T11:33:19Z
dc.date.issued	2004
dc.description	©2004. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in Histology and Histopathology. To access the final edited and published work see https://doi.org/10.14670/HH-19.69	es
dc.description.abstract	The response to lead nitrate has been assessed in two cell lines of renal origin. The range of toxic concentrations was determined by Neutral Red assay after 24-h of exposure. Morphological changes in the Buffalo Green Monkey (BGM) and VERO cell lines after exposure to subcytotoxic doses (1.38 mM and 1.04 mM, respectively) equivalent to EC10 (effective concentrations 10%) of lead nitrate were evaluated at the ultrastructural level by transmission microscopy. The most notable finding in treated cells was the presence of inclusion bodies in the form of irregular granules of varying size in both cytoplasm and lysosomes. Cell membrane integrity was not affected. The number of phagolysosomes and myeline figures associated to the inclusion bodies was higher than in the control cultures. We conclude that the phagolysosomic mechanism fails to digest this metal ion and the BGM and VERO renal cell lines can be considered as useful tools for toxicological studies involving lead nitrate.	es
dc.format	application/pdf	es
dc.format.extent	8	es
dc.identifier.citation	Histology and Histopathology, 19, 69-76 (2004)
dc.identifier.doi	https://doi.org/10.14670/HH-19.69
dc.identifier.issn	Print: 0213-3911
dc.identifier.issn	Online: 1699-5848
dc.identifier.uri	http://hdl.handle.net/10201/140141
dc.language	eng	es
dc.publisher	Sociedad Española de Histología e Ingeniería Tisular	es
dc.relation	This work has been supported by CICYT, Project BCM 2000-0284	es
dc.relation.publisherversion	https://www.hh.um.es/Abstracts/Vol_19/19_1/19_1_69.htm	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Vero	es
dc.subject	BGM	es
dc.subject	Lead	es
dc.subject	Cytotoxicity	es
dc.subject	Inclusion bodies	es
dc.subject.other	CDU::6 - Ciencias aplicadas	es
dc.title	Morphological characterization of renal cell lines (BGM and VERO) exposed to low doses of lead nitrate.	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	a0c84987-f342-48dc-973c-912958efee27
relation.isAuthorOfPublication.latestForDiscovery	a0c84987-f342-48dc-973c-912958efee27