High-resolution adaptive optics fluorescence imaging of retinal cell function

Merigan, William H.

Publication:
High-resolution adaptive optics fluorescence imaging of retinal cell function

dc.contributor.author	Merigan, William H.
dc.contributor.other	universidad de murcia	es
dc.date.accessioned	2020-11-03T15:50:27Z
dc.date.available	2020-11-03T15:50:27Z
dc.date.created	2011-06-09
dc.description.abstract	Abstract The long-term goal of our research is to manipulate and/or record the physiological activity of retinal neurons optically in the living eye, both in the high-acuity, perceptually capable macaque, and in the experimentally more tractable mouse. The fine spatial scale of adaptive optics imaging makes it attractive to use recently developed neuroscience methods, such as genetically encoded calcium indicators or light-gated channels, introduced into retinal neurons, to study the function of these cells. Such studies will be fruitful if they achieve: 1. high efficiency transduction of retinal cells in order that imaging or control of neurons can be achieved with light levels low enough to be consistent with retinal safety, 2. uniform transduction of the cells across the retina, which in macaque is hampered by the dense inner limiting membrane barrier, and 3. selective transduction of chosen cell types among bipolar, amacrine or ganglion cell classes. This talk will describe the progress we are making in reaching these goals. In collaboration with the Flannery laboratory we are developing viral vectors for intravitreal injection that are tailored to the unique requirements of the mouse and macaque retina. In collaboration with the Callaway lab we are exploring the use of viral vectors that are retrogradely transported from intracranial injections to retinal neurons. A major focus of this work is the development of cell-type selective transduction, which can be accomplished by focal injection of retino-recipient nuclei that are receive input from only a single type of retinal neuron.	es
dc.format	application/pdf	es
dc.format.extent	1	es
dc.identifier.uri	http://hdl.handle.net/10201/98102
dc.language	eng	es
dc.relation	Sin financiación externa a la Universidad de Murcia	es
dc.relation.ispartof	Engineering the Eye III	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject.other	CDU::6 - Ciencias aplicadas::61 - Medicina::617 - Cirugía. Ortopedia. Oftalmología	es
dc.title	High-resolution adaptive optics fluorescence imaging of retinal cell function	es
dc.type	info:eu-repo/semantics/other	es
dspace.entity.type	Publication	es