Publication: Clinical significance of stem cell marker CD133 expression in colorectal cancer
Authors
Wang, Bin-Bin ; Li, Zhi-juan ; Zhang, Feng-Feng ; Hou, Hai-Tao ; Yu, Jing-Kui ; Li, Feng
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
10.14670/HH-11-676
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info:eu-repo/semantics/article
Description
Abstract
Objective: CD133, a glycoprotein, is
expressed in different types of human stem cells and
tumor cells. Detection of altered CD133 expression in
colorectal cancer tissues could be useful as a marker for
the prediction of colorectal tumorigenesis, progression,
and prognosis. Methods: A total of 19 fresh and 145
paraffin-embedded tissue specimens from colorectal
cancer patients were obtained for detection of CD133
expression using flow cytometry and immunohistochemistry, respectively. The tumorigenic capacity of
tumor cells from 19 patients was assessed in nude mice.
Association of CD133 expression was then analyzed for
clinical significance. Results: The percentage of CD133-
positive (CD133+) tumor cell population ranged between
0.84% and16.75% (mean ratio=7.15%) of tumor cells in
the 19 freshly isolated tissue samples. CD133 expression
in tumor cells was associated with tumor lymph node
metastasis (9.81% vs. 3.22%; p=0.013) and poor tumor
differentiation (8.32% vs. 5.07%; p=0.043). In the 145
paraffin-embedded samples, CD133+ colorectal cancer
was also associated with local recurrence of
tumorigenesis (p=0.035) and distant metastasis
(p=0.017), while patients with over 5% CD133+ tumor
cells exhibited a decreased survival rate (p=0.001).
Multivariate COX analysis showed that the depth of
tumor invasion, histology, stages, lymph node
metastasis, and CD133 expression were all independent
prognosis factors for colorectal cancer (p=0.032, 0.011,
0.001, 0.002, and 0.030, respectively). Furthermore, as
few as 5,000 CD133+ colorectal cancer HCT116 cellswere sufficient to form tumor xenografts, whereas 1x105
CD133- tumor cells failed to develop tumor xenografts
in nude mice. Conclusions: CD133 expression is a useful
biomarker for prediction of colorectal cancer progression
and survival of patients.
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Citation
Histology and Histopathology, vol.31, nÂş3, (2016)
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