Effect of diflufenican on total carotenoid and phytoene production in carrot suspension‑cultured cells

Fraser, Paul D.; Almagro Romero, Lorena; Miras Moreno, Begoña; Pedreño García, María Ángeles; Sabater Jara, Ana Belén

Publication:
Effect of diflufenican on total carotenoid and phytoene production in carrot suspension‑cultured cells

dc.contributor.author	Fraser, Paul D.
dc.contributor.author	Almagro Romero, Lorena
dc.contributor.author	Miras Moreno, Begoña
dc.contributor.author	Pedreño García, María Ángeles
dc.contributor.author	Sabater Jara, Ana Belén
dc.contributor.department	Biología Vegetal
dc.date.accessioned	2025-05-15T09:56:24Z
dc.date.available	2025-05-15T09:56:24Z
dc.date.copyright	© 2018 Springer-Verlag GmbH Germany, part of Springer Nature
dc.date.issued	2018-08-06
dc.description.abstract	This work analyzes the efect of difufenican, an inhibitor of phytoene desaturase, on the gene expression profles of the biosynthetic pathway of carotenoids related with the production of these compounds in carrot cell cultures. The results showed that the presence of 10 µM difufenican in the culture medium increased phytoene levels, which was 493-fold higher than in control cells after 7 days of treatment but did not alter cell growth in carrot cell cultures. The maximal production of phytoene was reached with 10 µM difufenican after 7 days of incubation in the presence of light and with 30 g/L sucrose in the culture medium. Moreover, difufenican decreased the expression of phytoene synthase and phytoene desaturase genes at all the times studied. This difufenican-induced inhibition of phytoene desaturase gene expression in carrot cell cultures resulted in an increased production of phytoene. Our results provide new insights into the action of difufenican in carrot cell cultures, which could represent an alternative more sustainable and environmentally friendly system to produce phytoene than those currently used.
dc.format	application/pdf	es
dc.format.extent	10
dc.identifier.citation	Planta, 2019, Vol. 249, pp. 113–122
dc.identifier.doi	https://doi.org/10.1007/s00425-018-2966-y
dc.identifier.issn	Print: 0032-0935
dc.identifier.issn	Electronic: 1432-2048
dc.identifier.uri	http://hdl.handle.net/10201/154689
dc.language	eng	es
dc.publisher	Springer
dc.relation	Ministerio de Economía y Competitividad (no. BIO2017-82374-R), Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia (no.19876/GERM/15).	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s00425-018-2966-y
dc.rights	Attribution-NonCommercial-NoDerivates 4.0 International
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Carotenoids
dc.subject	Carrot suspension cultured cells
dc.subject	Difufenican
dc.subject	Phytoene
dc.title	Effect of diflufenican on total carotenoid and phytoene production in carrot suspension‑cultured cells	es
dc.type	info:eu-repo/semantics/article	es
dc.type.version	info:eu-repo/semantics/acceptedVersión
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