Publication:
Gasdermin D mediates a fast transient release of ATP after NLRP3 inflammasome activation before ninjurin 1-induced lytic cell death

Simple item page
dc.contributor.authorSchachter, Julieta
dc.contributor.authorGuijarro, Adriana
dc.contributor.authorAngosto-Bazarra, Diego
dc.contributor.authorPinilla, Miriam
dc.contributor.authorHurtado-Navarro, Laura
dc.contributor.authorMeunier, Etienne
dc.contributor.authorPerez-Oliva, Ana Belen
dc.contributor.authorSchwarzbaum, Pablo J
dc.contributor.authorPelegrín Vivancos, Pablo
dc.contributor.authorPinilla Marquinez, Míriam
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2025-02-20T13:10:07Z
dc.date.available2025-02-20T13:10:07Z
dc.date.issued2025
dc.description©2025. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This document is the published version of a Published Work that appeared in final form in Cell Reports. To access the final edited and published work see https://doi.org/ 10.1016/j.celrep.2025.115233
dc.description.abstractPyroptosis is a lytic cell death triggered by the cleavage of gasdermin (GSDM) proteins and subsequent pore formation by the N-terminal domain oligomerization in the plasma membrane. GSDMD is cleaved by caspase-1/-4/-5/-11 upon inflammasome activation and mediates interleukin (IL)-1β and IL-18 release. GSDMD pores favor ninjurin 1 (NINJ1)-induced plasma membrane rupture and cell death. Here, we demonstrate that GSDMD mediates early ATP release upon NLRP3 inflammasome activation independently of NINJ1, occurring before IL-1β release and cell death and constituting an early danger signal. The release of ATP is a transient signal terminated before the cells continue to permeabilize and die. The different N termini of GSDMA to -E are also able to release ATP and induce monocyte migration toward pyroptotic cells. This study reveals ATP release as an early and transient danger signal depending on GSDMD plasma membrane permeabilization, independently of the late stages of lytic cell death.es
dc.formatapplication/pdfes
dc.format.extent14es
dc.identifier.citationCell Reports 44(2)
dc.identifier.doihttps://doi.org/ 10.1016/j.celrep.2025.115233
dc.identifier.urihttp://hdl.handle.net/10201/150760
dc.languageenges
dc.publisherCell Presses
dc.relationMCIN/AEI/10.13039/501100011033, FEDER/Ministerio de Ciencia, Innovación y Universidades – Agencia Estatal de Investigación (grants PID2020-116709RB-I00 and RED2022-134511-T to P.P.), MCIN/AEI/10.13039/501100011033 and European Union «Next Generation EU/PRTR» (grant CNS2022-135105 to P.P.), Fundación Séneca (grant 21897/PI/22 to P.P.), the Instituto de Salud Carlos III (grants DTS21/00080 and AC22/00009 to P.P.), the EU Horizon 2020 project PlasticHeal (grant 965196 to P.P.), and an Invivogen-ANRT CIFRE PhD grant (Invivogen) to M.P. and E.M. L.H.-N. was supported by fellowship 21214/FPI/19 (Fundación Séneca, Spain) and AG fellowship PRE2021-100356 (Ministerio de Economía y Competitividad). J.S. was supported by Universidad de Murcia (R-745/2022) and Fundación Séneca (22192/IV/23).es
dc.relation.publisherversionhttps://www.cell.com/cell-reports/fulltext/S2211-1247(25)00004-Xes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPyroptosises
dc.subjectGasdermines
dc.subjectCell deathes
dc.subjectInflammationes
dc.subjectMacrophagees
dc.subjectExtracellular ATPes
dc.subjectInflammasomees
dc.titleGasdermin D mediates a fast transient release of ATP after NLRP3 inflammasome activation before ninjurin 1-induced lytic cell deathes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication547c4c2c-d906-4e1d-ae9a-0cdb40eef6d1
relation.isAuthorOfPublicationba71e300-bbe9-421f-82d9-f25730f55e54
relation.isAuthorOfPublication.latestForDiscovery547c4c2c-d906-4e1d-ae9a-0cdb40eef6d1
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PIIS221112472500004X.pdf
Size:
3.31 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos